Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (5): 645-649.doi: 10.3969/j.issn.1006-7795.2011.05.012

• 基础研究 • Previous Articles     Next Articles

Construction of parp-1 gene low expression in silica-associated malignant transformation of human bronchial epithelial cells by RNA interference

GUO Wei, SONG Shan-shan, TIAN Lin, GAO Ai, NIU Pi-ye, ZUO Xin, ZHU Zhong-hui, WU Hui-hui   

  1. Department of Occupational and Environmental Health, School of Public Health and Family Medicine, Capital Medical University, Beijing 100069, China
  • Received:2011-04-22 Revised:1900-01-01 Online:2011-10-21 Published:2011-10-21

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Abstract: Objective To construct poly(ADP-ribose) polymerase-1(PARP-1) gene low expression model in malignant transformation of human bronchial epithelial cell line(M-16HBE) induced by silica. Methods Parp-1 gene low expression model in M-16HBE was constructed by silencing of parp-1 with RNAi, and parp-1 mRNA expression was detected by RT-PCR. The effect of proliferation of cells was detected by MTT assay. Cell cycle was monitored by flow cytometry. Results The expression of parp-1 gene in transfected cells decreased(about 45% of normal cells) significantly, compared with normal cells and the cells transfected with empty vector(P<0.05). The expression of parp-1 gene in normal cells was equal to the cells transfected with empty vector(P>0.05). Compared with normal cells, the effect of proliferation of transfected cells was significantly increased(P<0.05); and the S phase ratio increased by 7.8%(P<0.05) and the G2 phase ratio decreased by 5.8%(P<0.05). Conclusion Parp-1 gene low expression model in M-16HBE was successfully constructed.

Key words: RNA interfering, poly(ADP-ribose) polymerase-1 gene, cell cycle

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