Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (5): 640-644.doi: 10.3969/j.issn.1006-7795.2011.05.011

• 基础研究 • Previous Articles     Next Articles

Effects of myocardial ischemia/reperfusion injury on the level of endogenous soluble receptor for advanced glycation end-products(sRAGE)

DONG Hong-wu1,2, GUO Cai-xia1, DU Feng-he1, WANG Hong-xia3, ZENG Xiang-jun3, ZHANG Li-ke3, TIAN Jun-ping1   

  1. 1. Department of Cardiology Medicine, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China;2. Department of Cardiology Medicine, Beijing Shijingshan Hospital, Capital Medical University, Beijing 100043, China;3. Department of Pathophysiology, School of Basic Medical Science, Capital Medical University, Beijing 100069, China
  • Received:2011-04-08 Revised:1900-01-01 Online:2011-10-21 Published:2011-10-21

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Abstract: Objective We evaluated the plasma levels of soluble receptor for advanced glycation end-products(sRAGE) of the cardiomyocytes in vivo undergoing ischemia/reperfusion(I/R) injury and the medium levels of sRAGE of cardiomyocytes in vitro undergoing hypoxia(H) or hypoxia followed by reoxygenation(H/R). Methods The rat model of myocardial I/R injury was induced by ligation of anterior descending coronary artery. All SD rats were randomly divided into two groups as follows: I/R group and sham group. The changes of heart rate(HR), left ventricular end-systolic pressure(LVESP) and the maximum rate change of left ventricular pressure(±LVdp/dtmax) in the different time points(10 minutes before ischemia, the end of 45 minutes' ischemia, the end of 60 minutes' reperfusion, the end of 120 minutes' reperfusion and the end of 180 minutes' reperfusion, for short BI-10, I-45, R-60, R-120, R-180) were observed and analyzed. Infarction size was measured by tri-phenyltetrazolium chloride(TTC) staining. The levels of sRAGE were determined by enzyme linked immunoabsorbent assay. The isolated cardiomyocytes were randomly divided into two groups as follows: H or H/R groups and control groups. Lactate dehydrogenase(LDH) release was determined by an enzyme activity assay kit(Nanjing Institute of Jiancheng Bioengineering, Nanjing, China). Results In the period of ischemia and reperfusion(I-45, R-60, R-120, R-180), the left ventricular function(HR, LVESP,±LVdp/dtmax) in I/R group decreased compared with control group. Within I/R group, the left ventricular function and the levels of sRAGE in the different time points(I-45, R-60, R-120, R-180) were decreased as compared with BI-10. There is no statistical differences in the sRAGE levels between H or H/R groups and control groups. Conclusion Our findings indicate that the levels of sRAGE were reduced after I/R injury and suggest that the molecules of sRAGE were involved in the pathophysiological of myocardial I/R injury.

Key words: soluble receptor for advanced glycation end-products, ischemia/reperfusion injury, myocardium

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