Abstract: Objective In recent reports,it has been indicated that 1,2,3,4,6-penta-O-galloyl-D-glucose(PGG)in Chinese herbal medicines is a natural active component which exhibited distinct anticancer,antidiabete and anti-obesity activities.Arapid,sensitive and reliable method using high-performance liquid chromatography is to be developed for determination of PGGin Radix Paeoniae Alba and Radix Paeoniae Rubra.Methods PGGwas extracted with 50% ethanol from Radix Paeoniae Alba and Radix Paeoniae Rubra.For the chromatographic separation and analysis of PGG,a reversed phase Diamonsil C₁₈ column(4.6 mm×250 mm,5 μm)was used at 25 ℃ with an isocratic mobile phase consisting of acetonitrile and 2.5% acetic acid solution(18∶82,V/V,pH=2.85).Flow rate was set at 1.0 mL/min.An ultraviolet detector was used and wavelength of detection was at 280 nm.Volume was 20 μL.Results The calibration curve for PGGwas linear over the range of 5 μg/mL～150 μg/mL(r =0.9999,n=5).PGGwas stable within 48 hours and showed good precision.The average recovery was 100.9%(RSD=3.57%,n=9).The contents of PGGin Radix Paeoniae Alba and Radix Paeoniae Rubra were 2.48 mg/g and 2.24 mg/g,respectively.Conclusion In the present work,a high-performance liquid chromatography method is developed for quantitative analysis of PGG in Radix Paeoniae Alba and Radix Paeoniae Rubra.The method is rapid,sensitive,reliable,and precise.It is applicable to determine PGGin Chinese herbal medicines.

Key words: PGG, HPLC, Radix Paeoniae Alba, Radix Paeoniae Rubra