Abstract: Objective To investigate the effect of Jianpibushenfang(JPBSF) on Ketamine-induced memory impairment in mice. Methods Memory errors of mice in training and retesting 24 hours later were measured by Step-down method. The memory acquisition impairments were induced by i.p Ketamine 20 and Anisodine 10 minutes before training in Step-down performance, respectively. Glutamate in mice brain was analyzed by automated amino acid assay. Results Memory errors of saline+ketamine group in retesting was more significant than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+ketamine group, JPBSF large dose+ketamine group and piracetam+ketamine group were significantly milder than that of saline+ketamine group(P<0.01). There was no difference in glutamate content in mice brain between JPBSF low dose+ketamine group and saline+ketamine group(P>0.05). Memory errors of saline+anisodine group in retesting was obviously more than that of saline+saline group(P<0.001), while memory errors of JPBSF low dose+anisodine group, JPBSF large dose+anisodine group and piracetam+ketamine group were significantly milder than that of saline+anisodine group(P<0.01). Conclusion JPBSF has improved ketamine and anisodine-induced memory acquisition impairment, but did not change the glutamate level in mice brain.

Key words: Jianpibushenfang, memory impairment, ketamine, glutamate