Abstract: The aim was to assess complement dependent cytotoxicity and change of IL-1 in hyperacute rejection of pig-to-human xenotransplantation.Cultural porcine vascular endothelial cells (PVECs) and human sera were used to set up an vitro model of hyperacute rejection of pig-to-human xenotransplantation.Various serums were divided into four groups: HS, HI HS, C1q-D, PS.Every serum was incubated with cultural primary porcine endothelial cells from 0.5 to 4 h, then supernatant fraction was removed to test LDH content, finally percent cytotoxicity was calculated.Morever, IL-1β in supernatant fraction was determined by ELISA.Result: 1) In the same time point, percent cytolysis of HS was higher significantly than that of control (P<0.01) and HI HS, C1q-D's percent cytolysis was similar to control's (P>0.05).As time prolonged, percent cytolysis of HS continued to increase ( P< 0.01).However, percent cytolysis of every group didn't increase after 3～4 h.2) In the group of HS, the contents of IL-1β were higher than those of control ( P< 0.01).Other groups' contents were similar to those of control ( P> 0.05).When reaction time prolonged, content in group HS went up and remained stable when reaction time arrived at 3～4 h.The results suggest that endothelial cells have an important role in the thrombosis in hyperacute rejection of pig-to-human xenotransplantation by influencing IL-1.

Key words: xenotransplantation, hyperacute rejection, vessel endothelial cell, interleukin-1