Table of Content

15 July 2004, Volume 25 Issue 3

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论著·基础研究

3D Reconstruction of Meridian Based on Digital Chinese Human-Female

Zhong Qin;Luo Shuqian

2004, 25(3):  281-283. 

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Based on the dataset of the female Virtual Chinese Human, a 3D atlas of three meridian lines on lower limbs was reported.Serial section images were marked according to the anatomic knowledge and then a 3D surface model was reconstructed.A multimedia presentation is also developed to relate this atlas to the PET brain functional images acupuncturing Zu-san-li point.

Direct Detection of Mitochondria by Polymerase Chain Reaction

Chu Jinhua;Xuan Dengfeng;Wu Ping;Zhan Shuhua;Li Guojun;Geng Rong

2004, 25(3):  284-285. 

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The aim was to establish a method for polymerase chain reaction (PCR) of mitochondria without mitochondrial DNA extraction.In high temperature, the membrane of mitochondrial was broken and intracellular DNA leaked out.DNA was analyzed by the PCR method.The amplified product was as much as the product which acquired from purified mitochondrial DNA.The studies suggest that this method is adapted to the common mitochondrial DNA amplified test.

Effect of Chloridion on Intracellular Potential in Crucian Carps' Mauthner Cell Evoked by Skin Stimulation

Zhang Lijuan;Tong Xuehong;Li Xiaoyi

2004, 25(3):  286-289. 

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In the present experiment, microelectrode was used to explore the physiological properties of the Mauthner cell with the stimuli of the skin.Direct stimulation of the skin evoked postsynaptic potentials (PSPs), which were stimulus strength-and frequency-dependent within some limits.The intracellular skin responses could be increased by passing hyperpolarizing currents to the M-cell through the Cl^- filled electrode.The results suggest that the afferent pathways from skin to M-cells contain both excited and inhibitory compotents, which interact each other and modulate the excitability of the M-cells together.

Liposomes as Carrier of Thrombolysis Medicine Against Thrombus

Wang Hongxia;Yang Hui;Hao Gang;Zeng Xiangjun;Lu Lingqiao;Jiang Ying;Zhu Xian;Yao Xinghai;Zhang Like

2004, 25(3):  290-293. 

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The aim was to observe the effects of liposomes containing thrombolysis dosage connected with blood platelet membrane glycoprotein as thrombolysis homing equipment on dissolving thrombus.The common abdomenal artery thrombus model was established with FeCl 3.Different thrombolysis dosage was from femoral vein: ①buffer(PBS), ②5.2×10⁴ IU/kg urokinase (UKl), ③15.6×10⁴ IU/kg urokinase, ④5.2×10⁴ IU/kg mixture of urokinase, liposomes and RGDS, ⑤ The mixture of liposomes encapsulating 5.2×10⁴ IU/kg urokinase and RGDS, ⑥ the coupler of liposomes and RGDS enwrapping 5.2×10⁴ IU/kg urokinase.Blood pressure, wet weight of thrombus and morphologic changes in UKh and U-L-R groups differed greatly from other groups.The results show liposomes as the carrier of medicine has better target anti-thrombus effect after RGDS modifies it.

Localization for Rabbit Femoral Venous and Arterious Thrombus

Dong Xiaoli;Li Liang;Wang Jue;Song Aili;Zhang Shunying;He Shida;Chen Tiejun

2004, 25(3):  294-297. 

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The aim was to evaluate the precious localization of femoral venous and arterious thrombus targeted by ^99mTc monoclonal antibody.Antibody labeled with ^99mTc-anti-D-dimer was administrated into femoral vein and artery of the rabbits that were subjected to the thrombotic operation.Using radioimmunoimaging technique, the distribution, blood flow and radio-specificity of ^99mTc-anti-D-dimer within thrombus were analyzed both in intact and thrombolized animals.Results: ^99mTc-anti-D-dimer was specifically concentrated in thrombus, not in clamp and normal side.In addition, no abnormal reaction resulted from the injection of ^99mTc-anti-D-dimer and no pathologic changes in main organs were found.Conclusion: the ^99mTc-labeled anti-D-dimer antibody will be one of potentially tools for experimental thrombus diagnosis.

Morphologic Observation of Effect of Mobile Telephone Microwave Radiation on Mouse

Liu Yu;Liu Guozhen;Dong Xiaoli;Song Aili;Chen Anyu

2004, 25(3):  298-301. 

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In order to explore the effect of mobile telephone microwave radiation on mouse, the expression of synthase(NOS) in hippocampus and cerebellum and the changes of primary organs of mouse was studied following exposure to the mobile telephone microwave radiation.SP immunohistochemical staining was employed to detect the expression of NOS; HE staining was used to observe histopathological changes.Compared with normal control group,the intensity of positive staining in cerebellum was increased after exposure to microwave radiation(450, 870, 2 450 MHz).The expression of NOS in hippocampus and the primary organs of mouse had no obvious change.The results indicate the increase of NOS expression in cerebellum possibly has effect on the function of cerebellum of mouse after exposure to microwave radiation, but further discussion and investigation is necessary.

Interleukin-2 Activity in Lymph Node During Experimental Spleen-deficiency Syndrome Combined with Gastric Ulcer in Rats

Chen Tian'e;Wang Xiuqin;Sun Haimei;Zeng Xiaobei;Li Baohong

2004, 25(3):  302-306. 

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The objective was to explore the functional changes of cellular immunity during experimental spleen-deficiency syndrome combined with gastric ulcer.Using immunohistochemistry, laser con-focal microscopy to examine the activity of IL-2 secreted by immunocytes in mesenteric lymph node.The results showed that the level of IL-2 were all higher in 4 d and 14 d in gastric ulcer group, but decreased during in spleen-deficiency group and group with spleen-deficiency and gastric ulcer.It indicates that the function of cellular immunity of the body will decrease when spleen-deficiency and spleen-deficiency combined with gastric ulcer syndrome developed to some degree.

Investigation of Pathogenesis in Acute Pancreatitis

Chen Ruifen;Zhao Min;Wang Min;Wang Xiaoping;Zhou Yijian;Wang Jue

2004, 25(3):  307-310. 

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Observations of pathologic morphology were made at the early phase of acute hemorrhagic and necrotic pancreatitis(AHNP), to investigate pathogenesis of acute pancreatitis.AHNP was induced in rats(30, weight 300～350 g) by intraductal administration of 5% sodium taurocholate.The pathologic morphologic changes of pancreatic tissue were examined by light microscope by HE and PTH and electron microscope after induced 5 min to 6 h.Main pathological changes: hemorrhage and necrosis appear in pancreas, fibrin thrombus, white thrombus and mixed thrombus in small vessels.With the development of the disease pathological changes were aggravated.The result suggests: AHNP is associated with self-digestion and microcirculation disturbance.

Protecting Effects of Quercetin on Myocardium Ischemia Repurfusion Injury in Rats

Cao Wenjun;Chen Ruifen;Liu Guozhen

2004, 25(3):  311-313. 

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Protective effects of quercetin on myocardium ischemia repurfusion injury were studied in rats.Wistar rats were divided into 6 groups: pseudo operation; ischemia reperfusion operation; ischemia reperfusion with quercetin 0.2 mg/kg, 1.0 mg/kg and 5.0 mg/kg; ischemia reperfusion with verapamil 1 mg/kg.The results showed that quercetin possesses protective effects on myocardium ischemia reperfusion injury in rats and its mechanism may be related to inhibition of lipid peroxidation of quercetin.

Effect of Almond Milk on Antioxidant Capacity in Cancer Patients

Liu Zhen;Tang Yuping;Liu Jing;Li Penggao;Wang Huiqin

2004, 25(3):  314-316. 

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The aim was to study the effect of Almond milk on antioxidant capacity in cancer patients.One hundred cancer patients were randomly divided into Group A, who administrated Almond milk from 3 weeks before the outset of trial phase to the end of the trail phase, and Group B, who administrated Almond milk from the beginning of trail phase to 3 weeks after the trail phase.SOD, LPO and GSH-Px were measured in both groups.The LPO level decreased evidently as compared to the beginning of trial phase(P<0.05).The SOD activity of subjects showed a significant enhancement at the beginning of trial phase.Almond milk can reduce the free radical damage in cancer patients.

Mitogenic Activity of Fibroblast in Earthworm

Cui Hong;Yu Peilan;Sun Lin;Zhang Zuxun;Zhang Haiyan;Li Weihong

2004, 25(3):  317-320. 

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By means of experiment, the affect of the tissue components purified from earthworm on the mitogenic activity of fibroblast was studied to investigate its pharmic mechanism in the wound healing.In the experiments, using acetate buffer the tissues components were purified from the earthworm, which was crude extract.A group of proteins were obtained from the crude extract of the earthworm through ammonium sulfate precipitation, ion exchange chromatography and gel filtration chromatography.The effect of the group of proteins on the mitogenic activity of fibroblast was investigated with MTT method.It was illustrated that the group of proteins of mitogenic activity was on the first peak of Sephadex-G100, and presented three bands in SDS polyacrylamide gel electrophoresis.The range of their molecular weighs were 57 000, 72 000, 87 000.It can be drawn that there are some kind of proteins in the tissue components of the earthworm that has mitogenic activity of fibroblast.

论著·临床研究

Relationship between Methylenete-trahydrofolate Reductase C677T gene Polymorphism and Hyperhomocysteinmia and Pulmonary Thromboembolism

Ye Yanping;Wang Chen;Liu Yanmei;Huang Xiuxia;Pang Baosen

2004, 25(3):  321-325. 

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The objective was to detect the relationship of methylenetetrahydrofolate reductase (MTHFR) C677T gene polymorphism and pulmonary thromboembolism(PTE) in Chinese Han nationality population and to investigate the possibility that hyperhomocysteinemia(HHc) is an independent risk factor related with pulmonary thromboembolism(PTE).Methods: 92 patients with PTE and 92 persons as controls, which were matched by age and sex, and analyzed the MTHFR C677T genotypes by the means of polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP), measured the serum homocysteine levels through the ways of Enzyme Linked Immuno Sorbent Assay(ELISA).Results:①The frequencies of the MTHFR gene C677T mutation TT genotype in PTE cases and controls were respectively 30.4% and 29.4%, the frequencies of T allele gene were 50% and 47%.There was no significant difference in the genotype distribution and allele frequencies between the two groups(P>0.05).The odds radios(OR) of TT genotype for PTE was 1.22(95%CI:0.59～2.51).This indicated that ① TT genotype didn't increase the risk of PTE.②The serum Hcy level in PTE group (17.1±9.39)μmol/L was significantly higher than that in control group(13.8± 7.4)μmol/L (P=0.034); HHc increased in risk of PTE.(odds radios= 3.67,95%CI:1.84～7.31);③The serum Hcy level in PTE group with CC,CT and TT genotype were (14.8±6.5)μmol/L,( 15.8±7.3)μmol/L,(21.5±11)μmol/L,versus (12.1±5.2)μmol/L,(11.9±7.0)μmol/L,(19.8±9.2)μmol/L in the control group.The serum Hcy level in PTE cases and control subjects with TT genotype was significantly higher that with CT or CC genotype in the same group(P<0.01).Indications: Hyperhomocysteinemia increase in risk of PTE, which may be an independent risk factor for PTE in Han nationality in China.MTHFR 677TT genotype is related to hyperhomocysteinemia ,but there is no direct link between MTHFR C677T gene polymorphism and PTE.

Comparison of Changes of Cerebral Blood Flow at Different Position in Cats with Brain Ischemia

Shi Guangzhi;Zhang Shuzhen;Zhang Shaodong;Luo Fang;Zhai Jing

2004, 25(3):  326-328. 

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The purpose was to observe the changes of cerebral blood flow at different position using the model of the cats with brain ischemia.The middle cerebral artery (MCA) of every cat was occluded after craniotomy firstly, The animals were divided into two groups monitoring cerebral blood flow (CBF) continuously by Laser Doppler flowmetry in two hours: Group 1 (n=6), at temporal cortex; Group 2 ( n=6), at occipital cortex.After the MCA was occluded, the CBF whether at temporal or at occipital cortex reduced quickly to 21.44% and 23.61% of the normal value consequently.The CBF decreased slowly in two hours and reduced to 15.74% and 9.96% at last.The CBF had no statistics difference in 0 h, 0.5 h, 1 h, 1.5 h, except at 2 h.The change of the CBF at temporal and occipital cortex has no statistics difference in cats with brain ischemia.

Effect of HCV Infection on HAART in HIV/AIDS Patients

Zhang Yonghong;Wu Hao;Jiang Yan;Chen Xinyue;Xiao Yao;Xu Lianzhi

2004, 25(3):  329-331. 

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The aim was to explore the impact of HCV infection to effect of HAART in HIV/AIDS patients.Patients were divided into two groups, HIV/HCV co-infection group and the group of HIV infection alone.Comparisons of the two groups were made on the change of immune function and HIV viral load at the points of pre-cure, month 3, month 6.There were no significant differences in immune function and HIV viral load at three points above between HIV/HCV co-infection and HIV alone(P> 0.05).But significant differences were found in the change of immune function from pre-cure to month 6.The rising of CD⁴⁺T was (47.87±48.42)cells/μL in HIV/HCV co-infection group, but (146.12±88.29)cells/μL in HIV infection alone (P=0.017).After cure of HAART the reconstruction of immune function in HIV/HCV co-infection patients is inferior to patients with HIV infection alone.

Expression of Nuclear Factor Kappa B(NF-κB) in Pneumonia of Severe Acute Respiratory Syndrome

Zhang Lijie;Song Chenzhao;Lang Zhenwei;Meng Xin;Zhang Shijie;Sun Lin;Li Junqiang

2004, 25(3):  332-334. 

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In order to study the pathological characteristics of lungs in severe acute respiratory syndrome (SARS) and the expression of nuclear factor kappa B (NF-κB) in lungs, investigate the relationship between the activation of NF-κB and damage of lungs, HE staining and immuno-histochemical staining (SP methods) were used to study 3 lungs of autopsy cases of SARS.Antibody against NF-κB p65 was used for immunohistochemical studies.The pathological changes were diffuse pulmonary interstitial inflammation and alveolar damages with apoptosis and desquamation of pneumocytes in alveolar spaces, hyaline-membrane formation, wider alveolar walls and predominant lymphocyte and mononuclear inflammatory infiltrates.Immunoreactivity to NF-κBp65 were all positive and located in plasma and nuclei of pneumocytes, macrophages and lymphocytes.This study indicates that the lungs of patients with SARS show acute injury, NF-κB plays an important role in it.

Possible Risk Factor for Death Patients with Severe Acute Respiratory Syndrome

Meng Yanying;Wang Shuzhao;Zhang Ke;Huang Chun

2004, 25(3):  335-337. 

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The aim was to explore the possible risk factor for death patients with Severe Acute Respiratory Syndrome.15 death SARS patients from March to June 2003 were analyzed.Results: Age: 23 to 81years old, average age were (50.7±18.8) years old, 7 patients were more than 50 years old.Underlying disease: 5 patients with diabetes, 5 patients with hypertension, 2 patients with chronic renal function failure and uraemia.T subset: CD⁴⁺ T lymphocyte decreased significantly at the first week of disease period and average CD⁴⁺T count were (248±82)cells/μL, average CD⁴⁺ T count were (181±128)cells/μL at second week and (125±46)cells/μL at third week, compared with group of cured patients, it had significantly difference(P<0.05).Chest image: chest image of 7 patients was apparent of pan-ground glass and consolidation in bilateral lung lobes.CK and CK-MB: average CK were ( 269.3± 399.9)U/L and CK-MB were (31.1±26.1)U/L.The adverse clinical outcome will be shown if SARS patient with older age, multi-underlying disease, rapid decrease in T subset, rapid progress of appearance of pan-consolidation in chest image, elevate of CK and CK-MB.

Dynamic Analysis of T Lymphocyte and Subset in 95 SARS Patients

Zhang Tiange;Zhang Ke;Wang Shuzhao;Guo Xinhui

2004, 25(3):  338-340. 

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The aim was to analyze the dynamic variation of T lymphocyte subset in 95 patients with severe acute respiratory syndrome (SARS).Among all 95 patients, 85 patients were survivor, 10 patients died of this severe disease.As for 85 survivor patients, the average CD⁴⁺T lymphocyte count and CD⁸⁺ T lymphocyte count were (325±190)cells/μL and (319±315)cells/μL, in 7th day of disease course, respectively, the ratio of CD⁴⁺T/CD⁸⁺ T was 1.25±0.71 which were distinctly lower than that of the normal Chinese peoples whose average CD⁴⁺T lymphocyte count were (727±255)cells/μL, CD⁸⁺ T (539±134)cells/μL, CD⁴⁺T/CD⁸⁺ T were 1.49(P= 0.001).14 days later of disease course, the immunological function of the SARS patients recovered gradually, the average CD⁴⁺T were (561±522)cells/μL, CD⁸⁺ T (370±271)cells/μL, CD⁴⁺T/CD⁸⁺ T were 1.68±1.11.21 days later, their immunological function became normal, the average number of CD⁴⁺T lymphocyte count were (675±448)cells/μL, CD⁸⁺ T lymphocyte count were (467±241)cells/μL, CD⁴⁺T/CD⁸⁺ T 1.48±0.68.While T lymphocyte subset of 10 dead cases appeared to decline in 7th day, 14th day and 21th day.first, their CD⁴⁺T lymphocyte count went down from (248±82)cells/μL in 7th day to (181±128)cells/μL in 14th day and (125±46)cells/μL in final.CD⁸⁺ T lymphocyte count from (233±115)cells/μL to (173±109)cells/μL and to (94±38)cells/μL in final.CD⁴⁺T/CD⁸⁺ T was 1.21±0.40, 1.17±0.45, 1.44±0.59.Conclusion: Activation of abnormal immune response were common in the early stage of SARS, it may be closely correlated to rapidly progression of this disease.T lymphocyte subset will recovery in the second week of disease course, However, it will have bad prognosis if T lymphocyte subset continues to go down without recovery.

G^- Bacteria Infection in Patients with Hepatic Disease

Zhang Wenzhong;Guo Qiuyan;Liu Haixia

2004, 25(3):  341-344. 

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To investigate the spectrum of bacteria isolated from hepatitis and characteristics of drug resistance and to evaluate the clinical preventive and therapeutic measures, the bacterial spectrum separated from blood/body fluids of patient with hepatitis were examined to estimate the ability of producing enzyme.And their drug resistance and clinical characteristics were analysed.Result of the G^- bacteria, Escherichia coli, Klebsiella pneumoniae and aeromonas strains were the major ones.The bacteria producing beta-lactamase were mainly from Escherichia coli and Klebsiella pneumoniae and their susceptibilities to antibiotic were lower than those of no enzyme product bacteria.The result indicates that antibiotics should be selected based on susceptibility.

Comparion of Bisoprolol and Carvidilol in Antihypertensive Effects and Reverse Left Ventricular Hypertrophy in Patients with Essential Hypertension

Li Xuewen;Hua Qi;Pi Lin;Gao Jing;Li Mei;Liu Rongkun

2004, 25(3):  345-348. 

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The aim was to investigate the effects of antihypertension and reverse left ventricular hypertrophy by carvedilol or bisoprolol in patients with mild to moderate essential hypertension.The forty cases of mild to moderate essential hypertension were selected for this random single-blind, paralleling controlled clinical study.Patients were randomized to take 12.5～25 mg carvedilol tablet once daily or bisoprolol 2.5～5 mg once daily if DBP was still in the range of 12.0～14.6 kPa(90～110 mmHg) after 2 week placebo baseline.Carvedilol group included 20 cases, Bisoprolol group included 20 cases, The course was 24 weeks, Blood pressure and heart rate were measured and symptoms and signs were recorded.At the end of placebo and in 24 weeks examined heart ultrasound, blood routine, serum glucose, blood lipid, hepatic function and renal function.SBP, DBP and heart rate of patients in two groups decreased obviously.There were significant differences between the two groups.Ventricular hypertrophy of carvedilol group improved than pretherapy.There were significant differences between the two groups.Carvedilol was well-tolerated with less side effects that concluded mild headache, tiredness, dizziness, slightly elevating of serum Glucose.Carvedilol can well treat the mild moderate essential hypertension effectively and safely by 12.5～25 mg once daily.

Relationship between Endothelial Nitric Oxide Synthase 4b/a Gene Polymorphism and Curative Effects of Valsartan in Hypertensive Patients

Hua Qi;Li Dongbao;Zou Haiqiang;Chen Biao;Pi Lin;Liu Rongkun

2004, 25(3):  349-352. 

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To investigate the response of patients with different endothelial nitric oxide synthase(eNOS) genotype (4b/a)to the treatment of angiotensin Ⅱ type 1 receptor antagonist (valsartan), 72 patients with hypertension were divided into two groups according to eNOS gene 4b/a polymorphism: aa+ab group (n=16)and bb group (n=56).All patients were treated with valsartan for 4 weeks and their blood pressure were measured.Effective rate of valsartan antihypertension in aa+ab group was 93.8%, but the effective rate in bb group was 64.3%.Curative effects of valsartan was significantly higher in aa+ab group than in bb group.It is suggested that eNOS 4b/a gene polymorphism can be used as one of adjustive indicators for predicting the effects of using angiotensin Ⅱ type 1 receptor antagonist in treatment of hypertensive patients.

Distribution and Drug Resistance of Bacteria in Patients with Nosocomial Lower Respiratory Tract Infection in Respiratory Ward

Dang Binwen;Zhang Jie;Shao Lili;Tang Mingzhong

2004, 25(3):  353-355. 

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The aim was to study the changes on the distribution and drug resistance of the bacteria in patients with the nosocomial lower respiratory tract infection (LRTI) in respiratory ward.The sputum's culture of the patients with the nosocomial LRTI in respiratory ward from 1998 to 2002 was studied with MIC methods.Results: 430 strains were isolated, 73% of which were Gram negative bacillus (GNB) and decreased gradually; 64% of GNB were non fermenters and increased.27% of which were Gram positive bacteria (GPB) and increased; 32% of GPB were S.aureus (SA) and increased, 26% of GPB were methicilin resistant SA (MRSA) and increased.Drug-resistant was growing, only few drugs, such as vancomycin to GPB (0) and Imipenem to GNB (17%), kept lower resistance.Conclusions: The distribution and drug resistance of the bacteria had been changing with time and antibiotics use, nosocomial pathogens have shifted away from easily treated bacteria toward more resistant bacteria.Few drugs, such as vancomycin and imipenem and so on, can be selected to treat nosocomial pathogens.Integrated management is a method to improve it.

Endoscopic Therapy and Clinical Characteristic of Dieulafoy Lesion

Dong Lifeng;Zhang Taichang;Jiang Dongwei

2004, 25(3):  356-358. 

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The aim was to investigate clinical characteristic of the Dieulafoy lesion and causation of gastrointestinal bleeding and.Patients were divided into 2 groups according to initial endoscopic treatment method.Thirty-seven cases of Dieulafoy lesion were endoscopic injection groups.Forty cases of upper GI bleeding from a peptic ulcer were control groups.Data were analyzed with respect to clinical and endoscopic characteristics as well as outcome.Endoscopic injection therapy was successful in 36 patients(97.3%).One patient had successful surgery after endoscopic therapy failed.Most patients with Dieulafoy lesion were elderly men 〔average (61.14±9.18) years〕 with severe acute upper gastrointestinal hemorrhage.There were significant differences between the 2 groups with respect to age ( P<0.01), hemorrhage values (P<0.01), localization of lesion ( P< 0.01), number of therapeutic endoscopic sessions (P<0.01), delitescent time (P<0.01), and number of units transfused (P< 0.01).There were no deaths due to bleeding and no endoscopic complications.Dieulafoy lesion occurs most frequently in elderly males with severe acute upper bleed gastroiatestinal hemorrhage.Many patients in this series (81.1%) had significant comorbidity, in particular, cardiovascular disease, hypertension, and diabetes.Endoscopic injection therapy of Dieulafoy lesion is safe, economical, effective, successful, and should be performed.There are no deaths due to bleeding and no endoscopic complications.

Soluble Intercellular Adhesion Molecule as Early Marker of Vasculopathy in Type 2 Diabetic Patients without Microalbuminuria

Mu Jun;Shi Bin;Zhuang Xiaoming

2004, 25(3):  359-362. 

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The aim was to test the hypothesis that endothelial dysfunction is already evident in normoalbuminuric with type 2 diabetes.Previous studies have shown that the development of microalbuminuria is associated with the onset of endothelial dysfunction in individuals with type 2 diabetes.Plasma soluble intercellular adhesion molecule(sICAM-1), soluble vascular cell adhesion molecule(sVCAM-1), vWF were measured by ELISA in 28 healthy control subjects(groupⅠ), 57 normoalbuminuria individuals with type 2 diabetes(groupⅡ), and 25 microalbuminuria individuals with type 2 diabetes(groupⅢ).Plasma sICAM-1 levels were significantly higher in the groupⅡ and groupⅢ than that in the groupⅠ(P<0.05).In contrast plasma sVCAM-1 and vWF levels were elevated only in the groupⅢ compared with groupⅡ and groupⅠ(P<0.05).Correlated analysis showed a significant positive correlation between sICAM-1 and blood pressure,triglyceride, vWF, sVCAM-1.The results suggest that plasma sICAM-1 levels might be implicated in the development of diabetic vasculopathy, and could predict the onset of endothelial dysfunction instead of plasma vWF levels.The measurement of plasma sICAM-1 levels in type 2 diabetic patients may be clinically useful for early discovering of endothelial dysfunction.

Factors Affecting Quality of Life in Patients with Advanced Cancer

Jia Aihua;Shao Jun;Ma Zhenshan

2004, 25(3):  363-365. 

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The aim was to discuss the factors that might affect the quality of life of patients with advanced cancer, and to find out the social-psychological demands of advanced cancer patients.A total of 268 advanced cancer patients and their family had been systematic intervened comprehensively.Based on pain-controlling, psychological guiding and nursing instruction were provided as assistance, and the overall quality of life of the patients were evaluated.After systematic intervention, quality of life of the patients had been improved significantly.But pain in 7% patients had not been effectively controlled.In addition, fatigue, nausea, vomiting and constipation were the most common symptoms in the patients.Negative moods were more common in patients who were not aware of their actual condition than the self-aware patients.Systematic intervention can improve the quality of life in patients.But currently, pain in cancer patients has not been controlled satisfyingly.Pain and other somatic symptoms are major factors to affect the mood of patients.Medical staff should evaluate and balance the demands of patients and their family from multiple aspects, to provide better service in consolatory treatment in advanced cancer patients.

Intrapulmonary Expression of Monocyte Chemotactic Protein-1 mRNA in Rats with Acute Necrotizing Pancreatitis

Zhu Bin;Sun Jiabang;Zhang Shuwen;Li Fei;Liu Shuang;Cui Yeqing;Sun Haichen

2004, 25(3):  366-369. 

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The aim was to investigate lung injury and intrapulmonary expression of MCP-1 mRNA in ANP.ANP was produced with 3.5% sodium taurocholate by the method of retrograde pancreatic-injection.Animals were sacrificed at 1,4,12 and 24 h after induction of pancreatitis.Intrapulmonary MCP-1 mRNA expression was assayed by RT-PCR.The following parameters were also measured: Serum lipases levels, pulmonary wet/dry weight ratio, histologic grading of AP and lung injury.Taurocholate pancreatitis showed necrosis, haemorrhage, and severe leucocyte infiltration in the pancreas, as well as alveolar septal thickening and leucocyte infiltration in the lung.There was intrapulmonary overexpression of the MCP-1 mRNA at the first hour and persisted up to the 24th h with the increase of serum lipases levels and pulmonary wet/dry weight ratio after pancreatitis induction.Expression of the MCP-1 mRNA correlated with score of the lung injury and pulmonary wet/dry weight ratio (P<0.05).The overexpression of MCP-1 mRNA is related to the production and severity of lung injury in ANP.

Change of IL-1 in Hyperacute Rejection of Pig-to-human Xenotransplantation

Jiao Wenjie;Chen Yuping;Li Wen;O Songlei;Zhang Zhitai;Wang Tiangyou

2004, 25(3):  370-373. 

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The aim was to assess complement dependent cytotoxicity and change of IL-1 in hyperacute rejection of pig-to-human xenotransplantation.Cultural porcine vascular endothelial cells (PVECs) and human sera were used to set up an vitro model of hyperacute rejection of pig-to-human xenotransplantation.Various serums were divided into four groups: HS, HI HS, C1q-D, PS.Every serum was incubated with cultural primary porcine endothelial cells from 0.5 to 4 h, then supernatant fraction was removed to test LDH content, finally percent cytotoxicity was calculated.Morever, IL-1β in supernatant fraction was determined by ELISA.Result: 1) In the same time point, percent cytolysis of HS was higher significantly than that of control (P<0.01) and HI HS, C1q-D's percent cytolysis was similar to control's (P>0.05).As time prolonged, percent cytolysis of HS continued to increase ( P< 0.01).However, percent cytolysis of every group didn't increase after 3～4 h.2) In the group of HS, the contents of IL-1β were higher than those of control ( P< 0.01).Other groups' contents were similar to those of control ( P> 0.05).When reaction time prolonged, content in group HS went up and remained stable when reaction time arrived at 3～4 h.The results suggest that endothelial cells have an important role in the thrombosis in hyperacute rejection of pig-to-human xenotransplantation by influencing IL-1.

Clinical Analysis of Secondary Epilepsies with Intracranial Arachniod Cyst

Cheng Yangquan;Han Jinsong;Xiong Xian

2004, 25(3):  374-376. 

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The purpose of this study was to determine the effect of surgical intervention of arachnoid cysts (ACs) on seizure outcome.The patients who has ACs with epilepsy treated in our hospital from Jan 1999 to Dec 2001 were reviewed .39 patients were reviewed.29 of the 39 patients were treated with operation (cyst removed or cyst-peritoneal shunt).In operating group 18 patients (72%) dealing with cysts removed were seizure free, 7 patients(28%) experienced seizure improvement.5 of 10 patients treated with anticonvulsant drugs (50%) remained unchanged, 5 of 10 patients improved.The successful outcome of the above cases suggests that removing cyst, fenestration, bipolar coagulation are likely to prove an effective method.

Acute Apoplexy Initiated by Residual Tumors of Large Pituitary Adenoma Extended to Suprasellar Region Following Operation

Yao Hongxin;Zhao Jizong

2004, 25(3):  377-379. 

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Residual tumors of three elder patients with large pituitary adenoma extended to the suprasellar region stroke acutely following operation were reported.Indication: Two men died after they were treated conservatively.One survived after reoperation.The large pituitary adenoma should undergo transcranial surgery, to ensure decompress on the hypothalamus and unblock cerebral spinal fluids circulation.If the diagnosis of pituitary apoplexy is established by computerized tomographic scans, emergent reoperation is needed.

Surgical Treatment of Late Posttraumatic Epilepsy

Zhang Kai;Zhang Jianguo;Sun Zhenrong

2004, 25(3):  380-382. 

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Clinical documents of forty-five late posttraumatic epilepsy cases were summarized.Surgical treatment was adopted in all of the cases.Detailed preoperative evaluation was done before the operation.ECoG monitoring was used during the operation.The surgical approaches included resection of the epileptogenic foci, anterior temporal lobectomy, anterior temporal lobectomy and hippocampectomy, bipolar coagulation of the cortex, corpus callosotomy.The follow-up results showed seizures of these patients improved evidently.According to Engel's grading, 23 cases were grade Ⅰ, 15 cases were grade Ⅱ, 7 cases were grade Ⅲ and no cases were grade Ⅳ.Surgical treatment is highly recommended in late posttraumatic epilepsy patients if systematic medical treatment fails.

Effect of Intraoperative Continuous Nimodipine Infusion on Systemic Hemodynamics in Aneurysm Surgery

Han Ruquan;Lu Zheng;Wang Baoguo;Wang Enzhen;Wang Shuo;Zhao Jizong

2004, 25(3):  383-386. 

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The aim of this study was to evaluate the effect of intraoperative continuous nimodipine infusion on systemic hemodynamics in intracranial aneurysm surgery.Thirty consecutive patients undergoing intracranial aneurysmal surgery without a preexisting cerebral ischemia were prospectively randomized into one of two groups: Isoflurane maintained anesthesia (group A, n=15) and nimodipine + isoflurane maintained anesthesia (group B, n=15).The patients in group B were given nimodipine infusion continuously 20 μg/(kg·h) after induction of anesthesia and anesthetized with 1 MAC isoflurane.The patients in group A were maintained with 1 MAC isoflurane anesthesia during the whole procedure.Systemic hemodynamics were monitored intraoperatively.Mean arterial pressure (MAP) were lower significantly in group B than that in group A, but MAP were kept in clinical safe range.Heart rates were kept stable.However, cardiac output, cardiac index, myocardial contract acceleration increased significantly in group B, and systemic vascular resistance decreased after nimodipine infused.Conclusion: Systemic hemodynamics during intraoperative continuous nimodipine 20 μg/(kg·h) infusion combined with 1 MAC isoflurane anesthesia are in clinical safe range.

Clinical Comparison of Norplant and Vcu200

Meng Fan;Xie Zheng;Gong Quan;Wu Minghui

2004, 25(3):  387-390. 

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The aim was to compare the effectiveness, the rates of side effects, discontinuation and menstrual bleeding patterns in Norplant and IUD.A randomized and clinical trial was done.The users of Norplant and Vcu200 were 315 and 320, respectively.The rates of continuation and discontinuation between two groups were compared by life table method and log-rank Chi-Square method.MDS recommended by WHO was used to analyze the menstrual bleeding patterns.Results: The cumulative continuation rate of Norplant and Vcu200 users were 80.07% and 84.42%, respectively.There was no statistic difference.The cumulative discontinuation of pregnancy, menstrual problem, other medical problem and contraceptive advice problem were 0.35%, 13.37%, 6.12%, 0.46% in Norplant users and 9.50%, 1.71%, 0.37%, 4.40% in Vcu200 users.The analysis of menstrual bleeding patterns showed the rate of irregular bleeding in Norplant users was 43.1%～57.6%.The rate of “normal patterns” was 12.9%～32.7% after six months using Norplant.The rate of “normal patterns” was 54.8%～60.2% in Vcu200 users.There was similar continuation rate between Norplant and Vcu200 for five years.In Norplant users the rate of pregnancy was lower, but the rate of irregular bleeding was higher.There are advantage and disadvantage between Norplant and Vcu200, respectively.

Analysis of 36 Cases with Classic Intrafacial SEMM Hysterectomy

Wen Qiaoying;Han Dan;Li Bing

2004, 25(3):  391-393. 

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The aim was to evaluate the indications, procedure and clinical value of classic intrafacial SEMM hysterectomy(CISH).Thirty-six women with benign gynecological disorders which needed uterine resection but remained cervix between March 2001 and July 2002 were assigned to a route of CISH on the basis of uterine size and score in operation.Operation time, blood loss, associated procedures, hospital stay and postoperative recovery were observed.CISH was successful in 97.2%(35/36) of women assigned to these groups.Transabdominal operation was performed in only 1 of 36 patients(2.78%).The average operating time was 103 min; Blood loss (50～150 mL) was lower than during conventional abdominal hysterectomy.The average hospital stay was 6 days.There were no complications found in all patients.The advantages of CISH are its minimum possibility of injury, reduced blood loss and a fast recovery period.Integrity of pelvic floor, vagina and cervix are maintained as far as possible with focus removal, thus preventing the subsequent development of cervix stump carcinoma.Therefore, CISH may be an optimal technique for benign uterine disease.

Endocervical Cytology in Diagnosis of Endometrial Carcinoma

Xu Xiaohong;Liu Yue;Pan Jing;Deng Xiaohong

2004, 25(3):  394-396. 

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To determine the value of endocervical cytology in detecting endometrial carcinoma, endocervical cells were assessed by Ori Endocervical Brush TM for 1 093 patients who accepted fractioned curettage.Cytodiagnostic results were compared with the histodiagnostic results, which were regarded as the golden standard.The achieving efficiency of endocervical brush smears and endocervical curettage's (ECCs) were 82.5% and 17.0% respectively.Of the 60 cases with endometrial carcinoma, 48 cases accepted staging operation, and 14 cases had cervical involvement.In judging the cervical involvement, the sensitivity and specificity of endocervical smears were 71.4% and 82.4%, respectively.While in ECCs, there were 50.0% and 79.4%, respectively.The cases reported as positive by endocervical cytology mostly have higher stage, higher grade and special histological type, which is useful in preoperative staging and deciding the type of operation.

Clinical Analysis of 35 Eclampsia Cases

Jin Liping;Jia Xiaofang;Dai Jiru;Wangrui;Zhu Xian

2004, 25(3):  397-399. 

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The aim was to investigate the methods of reducing the incidence of eclampsia and the managements of serious complications induced by eclampsia.A retrospective review was carried out on 35 eclampsia women admitted in our hospital from Jan 1988 to Dec 2002.The results showed that the incidence of eclampsia was 0.18% in the past 15 years, which revealed a declined tendency.2 cases of maternal death, the mortality was 5.71%; 10 cases of perinatal death, the mortality was 26.32%.The results indicated that intensive routine prenatal care, diagnosis and treatment of PIH in time, and paying more attention on postpartum management could all effectively reduce the incidence of eclampsia.The attendance of other departments is very important in treating the serious complications induced by PIH and in improving the maternal and neonatal prognosis.

Bacteriological Features of Patients with Chronic Periodontitis and Type-2 DM

Zeng Hongyan;Yang Shenghui

2004, 25(3):  400-402. 

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Bacteriological changes of subgingival flora of patient with chronic periodontitis and type-2 DM(diabetes mellitus) were researched by germ cultivation and PCR method in the thesis.Samples were taken from 42 patients with chronic periodontitis and type-2 DM, and 21 normal people.An isolated cultivation of subgingival plaque samples was performed by a anaerobe non-selected media, the Actinomyces, Fnusbacterium nucleatum, Eikenella corrodens, A.actinomycetemcomitans, capnocytophaga and Streptococcus spp were performed by selective media.The P.intermedia(P.i) of the black colony in the non-selective media was detected by PCR.The quantity of germs was recorded.It was discovered that the major germ of subgingival flora of type-2 DM patients with chronic periodontitis was anaerobe, the superiority germ was black germs, P.i, Fnusbacterium nucleatum and capnocytophaga.Statistical analysis of correlation between FPG、GHb and amount of black germs, capnocytophaga was made.It shows that chronic periodontitis is correlated to type-2 DM.