Intrapulmonary Expression of Monocyte Chemotactic Protein-1 mRNA in Rats with Acute Necrotizing Pancreatitis

Abstract

Abstract: The aim was to investigate lung injury and intrapulmonary expression of MCP-1 mRNA in ANP.ANP was produced with 3.5% sodium taurocholate by the method of retrograde pancreatic-injection.Animals were sacrificed at 1,4,12 and 24 h after induction of pancreatitis.Intrapulmonary MCP-1 mRNA expression was assayed by RT-PCR.The following parameters were also measured: Serum lipases levels, pulmonary wet/dry weight ratio, histologic grading of AP and lung injury.Taurocholate pancreatitis showed necrosis, haemorrhage, and severe leucocyte infiltration in the pancreas, as well as alveolar septal thickening and leucocyte infiltration in the lung.There was intrapulmonary overexpression of the MCP-1 mRNA at the first hour and persisted up to the 24th h with the increase of serum lipases levels and pulmonary wet/dry weight ratio after pancreatitis induction.Expression of the MCP-1 mRNA correlated with score of the lung injury and pulmonary wet/dry weight ratio (P<0.05).The overexpression of MCP-1 mRNA is related to the production and severity of lung injury in ANP.

Key words: acute necrotizing pancreatitis, animal model, lung injury, monocyte chemotactic protein-1(MCP-1), gene expression

CLC Number:

R576

Zhu Bin;Sun Jiabang;Zhang Shuwen;Li Fei;Liu Shuang;Cui Yeqing;Sun Haichen. Intrapulmonary Expression of Monocyte Chemotactic Protein-1 mRNA in Rats with Acute Necrotizing Pancreatitis[J]. Journal of Capital Medical University, 2004, 25(3): 366-369.

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Intrapulmonary Expression of Monocyte Chemotactic Protein-1 mRNA in Rats with Acute Necrotizing Pancreatitis

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