Abstract: Objective To investigate the genetic association of adiponectin gene (ADIPOQ) with coronary artery disease in type 2 diabetes mellitus. Methods A 2-step study was conducted. Firstly, the pilot study included bio-information analysis, sequencing the 2^nd exon regions in 20 T2DM complicated with CAD patients and contrasting to reference database to screen the candidate single nucleotide polymorphisms(SNPs). Secondly, the replication study was based on case-control study design. Of 680 type 2 diabetes mellitus patients, 213 individuals with coronary artery disease(CAD) from Beijing Anzhen Hospital and 467 controls from Beijing Hospital were recruited in our study. The polymerase chain reaction-high resolution melting(PCR-HRM) assay was carried out to genotype all the subjects for the candidate SNPs in ADIPOQ gene. Results In pilot study, 5 SNPs were screened, including 17366568G/A, 9882205G/A, 2241767A/G, 1501299G/T, 2241766T/G. Polymorphisms of rs2241766T/G(+45T/G) and rs9882205G/A were associated with risk of CAD[OR and 95% CI were 2.78(1.20~8.65) and 3.9(1.78~13.61), P<0.05]. In replication study, rs2241766 polymorphism was not associated with CAD in type 2 diabetes mellitus patients. Conclusion rs2241766 polymorphism may not be associated with coronary artery disease in type 2 diabetes mellitus.

Key words: ADIPOQ gene polymorphisms, type 2 diabetes mellitus, coronary heart disease