Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (2): 208-213.

Previous Articles     Next Articles

Changes of Membrane Potential in Pulmonary Smooth Muscle Cell Isolated from Chronic Thromboembolic Pulmonary Hypertension Patients

LI Jing1,2, WANG Chen2, LIU Jie1,2, LI Ji-feng1,2,3, LIU Yan2,4, GU Song2,4, YANG Yuan-hua2,3, ZHAI Zhen-guo2,3, GAN Hui-li5, WANG Jun1,2*   

  1. 1. Department of Physiology, School of Basic Medical Sciences, Capital Medical University; 2. Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing Institute of Respiratory Medicine; 3. Department of Respiratory and Critical Care Medicine, Beijing Chaoyang Hospital, Capital Medical University; 4. Department of Cardiac Surgery, Beijing Chaoyang Hospital, Capital Medical University; 5. Department of Cardiac Surgery, Beijing Anzhen Hospital, Capital Medical University
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-04-21 Published:2011-04-21
  • Contact: WANG Jun

PDF

310

Cited

1

Abstract:

Objective To investigate the method of isolating and culturing the pulmonary artery smooth muscle cells(PASMCs) from the endarterectomized specimens of CTEPH patients and to observe their electrophysiological characteristics.
Methods The endarterectomized tissues of CTEPH patients undergoing pulmonary thromboendarterectomy(CTEPH-PAMSCs) and normal lung tissues of bronchogenic carcinoma or bullae patients(normal-PASMCs) were collected, the PASMCs were isolated by enzymatic method and identified by SM-α-actin. Patch clamp technique was used to measure the cell’s membrane potential and action potential.
Results Human PASMCs from normal lung tissues and CTEPH patients’ tissues were successfully isolated and cultured that was confirmed by SM-α-actin staining. Compared with PASMCs of normal control, the membrane potential in CTEPH-PASMCs was more depolarized(-38.12mV±2.28 mV, n=10 vs -21.05 mV±2.20 mV, n=11, P<0.001) and the action potential duration at 50%, 75%, 90% were all prolonged(APD50: 0.100 s±0.016 s vs 0.185 s±0.035 s, APD75: 0.150 s±0.024 s vs 0.277 s±0.053 s, APD90: 0.180 s±0.028 s vs 0.333 s±0.064 s, P<0.05).
Conclusion The results suggested that the PASMCs in CTEPH patients has a more depolarized resting membrane potential and a prolonged action potential duration, which might be important factors determining the calcium influx via the voltage-gated calcium channel in pulmonary vascular remodeling.

Key words: chronic thromboembolic pulmonary hypertension, patch clamp, membrane potential, action potential

CLC Number: