Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (2): 203-207.

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Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

ZHANG Qian^1,2,3, LIU Jie^1,2,3, ZHANG Zhi-fei^1,2,3, LIN Yuan^1,2,3, WANG Jun^1,2,3*, WANG Chen^1,2,3*

1. Department of Respiratory and Critical Care , Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing Institute of Respiratory Medicine, Beijing Chaoyang Hospital, Capital Medical University; 2. Department of Physiology, School of Basic Medical Sciences, Capital Medical University; 3. Faculty of Respiratory, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-21 Published:2011-04-21
Contact: WANG Jun, WANG Chen

Abstract

Abstract:

Objective To explore the vasoconstrictive effect and mechanism of thrombin on pulmonary artery and thoracic aorta of rats.
Methods The pulmonary artery and thoracic aorta ring of rat were isolated in vitro according to the procedures routinely used. Vascular constriction tension recordings were accomplished by using Powerlab biology signal collection system. RT-PCR method was used to detect the mRNA expression of thrombin receptor in vascular rings.
Results The pulmonary artery and thoracic aorta rings were constricted by thrombin which was independent on the extracellular calcium. This effect was similar to that of the TRAP6, a thrombin receptor agonist. The mRNA expression of thrombin receptor-1 can be detected in the pulmonary artery and thoracic aorta.
Conclusion Thrombin induced pulmonary artery and thoracic aorta ring constriction was independent of extracellular calcium and might be mediated by thrombin receptor 1(PAR1).

Key words: thrombin, thrombin receptor-1, thoracic aorta, pulmonary artery, vasoconstriction

CLC Number:

R 331.3⁺3

ZHANG Qian;;LIU Jie;;ZHANG Zhi-fei;;LIN Yuan;;WANG Jun;;WANG Chen;. Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism[J]. Journal of Capital Medical University, 2011, 32(2): 203-207.

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Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

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