Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

doi:10.3969/j.issn.1006-7795.2011.02.008

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (2): 203-207.doi: 10.3969/j.issn.1006-7795.2011.02.008

Previous Articles Next Articles

Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

ZHANG Qian^1,2,3, LIU Jie^1,2,3, ZHANG Zhi-fei^1,2,3, LIN Yuan^1,2,3, WANG Jun^1,2,3*, WANG Chen^1,2,3*

1. Department of Respiratory and Critical Care , Beijing Key Laboratory of Respiratory and Pulmonary Circulation, Beijing Institute of Respiratory Medicine, Beijing Chaoyang Hospital, Capital Medical University; 2. Department of Physiology, School of Basic Medical Sciences, Capital Medical University; 3. Faculty of Respiratory, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2011-04-21 Published:2011-04-21
Contact: WANG Jun, WANG Chen

Abstract

Abstract:

Objective To explore the vasoconstrictive effect and mechanism of thrombin on pulmonary artery and thoracic aorta of rats.
Methods The pulmonary artery and thoracic aorta ring of rat were isolated in vitro according to the procedures routinely used. Vascular constriction tension recordings were accomplished by using Powerlab biology signal collection system. RT-PCR method was used to detect the mRNA expression of thrombin receptor in vascular rings.
Results The pulmonary artery and thoracic aorta rings were constricted by thrombin which was independent on the extracellular calcium. This effect was similar to that of the TRAP6, a thrombin receptor agonist. The mRNA expression of thrombin receptor-1 can be detected in the pulmonary artery and thoracic aorta.
Conclusion Thrombin induced pulmonary artery and thoracic aorta ring constriction was independent of extracellular calcium and might be mediated by thrombin receptor 1(PAR1).

Key words: thrombin, thrombin receptor-1, thoracic aorta, pulmonary artery, vasoconstriction

CLC Number:

R 331.3⁺3

ZHANG Qian, LIU Jie, ZHANG Zhi-fei, LIN Yuan, WANG Jun, WANG Chen, . Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism[J]. Journal of Capital Medical University, 2011, 32(2): 203-207.

0
/ Save 0 / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2011.02.008

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2011/V32/I2/203

[1]	Deng Hui, Wang Yong, Chen Xiaolan, Qin Chong, Pan Lei. Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats [J]. Journal of Capital Medical University, 2021, 42(4): 596-600.
[2]	Sha Yuhui, Gao Yang, Liu Jie, Qi Xianmei, Han Lulu, Wang Wang. FSTL1 promotes proliferation, adhesion and tube formation of pulmonary artery endothelial cells [J]. Journal of Capital Medical University, 2020, 41(1): 80-86.
[3]	Bai Lu, Bai Junqing, Wang Guoli. Early experimental diagnosis for deep vein thrombosis after traumatic hip arthroplasty in old and middle-aged patients [J]. Journal of Capital Medical University, 2016, 37(2): 223-227.
[4]	Guo Huan, Wang Wang, Sun Ran, Liu Jie, Wang Jun. Effects of hypoxia on the function of smooth muscle cells from different orders of pulmonary arteries [J]. Journal of Capital Medical University, 2014, 35(2): 231-236.
[5]	MAO Ting, WANG Yuexiu, LIU Jie, LI Jing, WANG Jun. Sildenafil inhibits hypoxia-induced voltage-dependent potassium channels downregulation in human pulmonary artery smooth muscle cells [J]. Journal of Capital Medical University, 2013, 34(2): 224-231.
[6]	KAN Ying, WANG Tie. Comparison between pulmonary ventilation/perfusion scintigraphy and CT pulmonary angiography at different levels of embolus [J]. Journal of Capital Medical University, 2013, 34(1): 23-28.
[7]	WANG Cong;;WANG Chen;WANG Yue-xiu;;LIU Jie;LI Ji-feng;;WANG Jun;. Endothelin-1 Promotes Human Pulmonary Artery Smooth Muscle Cells Proliferation by Up-regulation of Capacitative Ca²⁺ Entry [J]. Journal of Capital Medical University, 2009, 30(2): 161-166.
[8]	LI Ji-feng;;WANG Cong;;LIU Jie;WANG Yue-xiu;;WANG Jun;WANG Chen;. Hypoxia Promotes Human Pulmonary Artery Smooth Muscle Cells Proliferation Through Ca²⁺-NFAT Pathway [J]. Journal of Capital Medical University, 2009, 30(2): 172-176.
[9]	Li Yuanhong;Zhang Hongyu;Pang Baosen;Chen Yangyu;Mao Yanling;Ma Li;Huang Xiuxia;Xin Ping;Zhang Haiyan. The Abnormalities of Coagulating and Fibrinolytic System and the Effects of Pentoxifylline on Coagulating and Fibrinolytic Function in Experimental Obstructive Pulmonary Emphysema in Rats [J]. Journal of Capital Medical University, 2007, 28(5): 627-631.
[10]	Wang Qi;Zheng Yuanyuan;Zhang Weiyuan. Clinical Significance of Antithrombin-Ⅲ and P-selectin Changes in Normal Pregnancy [J]. Journal of Capital Medical University, 2007, 28(5): 655-657.
[11]	Guan Jian;Wang Guogan;Zhu Jun. The Direct Thrombin Inhibitors and Their Clinical Application [J]. Journal of Capital Medical University, 2007, 28(4): 462-466.
[12]	Zhang Kuimei;Yang Yi;He Shida. Clinical Significance of Variation of Serum Lipids Levels and Marker of Thrombin Activation in Patients with Diabetics [J]. Journal of Capital Medical University, 1998, 19(3): 260-261.
[13]	Gao Guojing;Hua Qi;He Shida. Clinical Significance of Serum Lipoprotein,Apolipoprotein and Lipid Levels and Marker of Thrombin Activation in Patients with Essential Hypertension [J]. Journal of Capital Medical University, 1997, 18(4): 348-349.
[14]	Jiao Xiaohui;Chen Xiaoliang;Han Daying. Protective Action of the Vescular Endothelial Cells on Tension of the Smooth Muscle [J]. Journal of Capital Medical University, 1994, 15(2): 100-102.
[15]	Liu Ping;Chen Xiaoliang;Han Daying. The Relaxant Effect of Bear Bile on the Isolated Aorta Thorcalis of the Rat [J]. Journal of Capital Medical University, 1993, 14(1): 1-4.

Vasoconstriction Effect of Thrombin on Pulmonary Artery and Thoracic Aorta ofRats and Its Possible Mechanism

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles 0

Metrics

Comments