Role of histamine in regulation of barrier function in human keratinocytes

doi:10.3969/j.issn.1006-7795.2015.04.018

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (4): 604-609.doi: 10.3969/j.issn.1006-7795.2015.04.018

Previous Articles Next Articles

Role of histamine in regulation of barrier function in human keratinocytes

Duan Su^1,3, Jiao Jian^2,3, Zhang Luo^1,2,3

1. Department of Allergy, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China;
2. Department of Otolaryngology Head and Neck Surgery, Beijing Tongren Hospital, Capital Medical University, Beijing 100005, China;
3. Beijing Key Laboratory of Nasal diseases, Beijing Institute of Otolaryngology, Beijing 100005, China

Received:2015-06-03 Online:2015-08-21 Published:2015-07-17
Supported by:
This study was supported by The 12th Five-year Science and Technology Support Project(2014BAI07B04), the Program for Changjiang Scholars and Innovative Research Team(IRT13082), Natural Science Foundation of Beijing(7131006), Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding Support(ZYLX201310).

Abstract

Abstract:

Objective We try to investigate the role of histamine in regulation of barrier function of human keratinocytes by testing the changes of trans-epithelial resistance(TER) and paracellular flux of primary human keratinocytes in vitro stimulated by histamine. Methods We cultured primary human keratinocytes from healthy and atopic dermatitis(AD) individuals in the presence and absence of histamine, histamine receptor blocker for 96 hours in air-liquid interface(ALI) culture systems. The epithelial barrier function was evaluated by measuring TER and FITC-Dextran paracellular flux. Results Histamine decreased the barrier integrity of human keratinocytes in ALI cultures by impairing TER increase and increasing the paracellular flux to Fitc-Dextran in a dose- and time-dependent manner. There was a statistically significant difference between histamine stimulated group and un-stimulated group. Histamine 1 receptor(H1R) antagonist could block the effect of histamine to TER and Ca²⁺ ionophore ionomycin could induce the similar effect to TER with histamine with statistical significance. Conclusion Our findings that histamine impairs human keratinocytes barrier function might contribute to the pathogenesis of chronic skin inflammatory diseases such as AD and might indicate a possibility for clinical therapeutic strategy.

Key words: histamine, skin keratinocytes, barrier function

CLC Number:

R751

Duan Su, Jiao Jian, Zhang Luo. Role of histamine in regulation of barrier function in human keratinocytes[J]. Journal of Capital Medical University, 2015, 36(4): 604-609.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.04.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I4/604

References

[1] Jurzak M, Adamczyk K. Influence of genistein on c-Jun, c-Fos and Fos-B of AP-1 subunits expression in skin keratinocytes, fibroblasts and keloid fibroblasts cultured in vitro[J]. Acta Pol Pharm, 2013,70(2):205-213.
[2] Hoffjan S, Stemmler S. On the role of the epidermal differentiation complex in ichthyosis vulgaris, atopic dermatitis and psoriasis[J]. Br J Dermatol, 2007,157(3):441-449.
[3] Shukuwa T, Kligman A M. Disaggregation of corneocytes from surfactant-treated sheets of stratum corneum in hyperkeratosis on psoriasis, ichthyosis vulgaris and atopic dermatitis[J]. J Dermatol, 1997,24(6):361-369.
[4] García-Pérez M E, Allaeys I, Rusu D, et al. Picea mariana polyphenolic extract inhibits phlogogenic mediators produced by TNF-α-activated psoriatic keratinocytes: impact on NF-κB pathway[J]. J Ethnopharmacol, 2014,151(1):265-278.
[5] Omori-Miyake M, Yamashita M, Tsunemi Y, et al. In vitro assessment of IL-4- or IL-13-mediated changes in the structural components of keratinocytes in mice and humans[J]. J Invest Dermatol, 2014,134(5):1342-1350.
[6] McCandless E E, Rugg C A, Fici G J, et al. Allergen-induced production of IL-31 by canine Th2 cells and identification of immune, skin, and neuronal target cells[J]. Vet Immunol Immunopathol, 2014,157(1-2):42-48.
[7] Wark P A, Johnston S L, Bucchieri F, et al. Asthmatic bronchial epithelial cells have a deficient innate immune response to infection with rhinovirus[J]. J Exp Med, 2005,201(6):937-947.
[8] Hackett T L, Singhera G K, Shaheen F, et al. Intrinsic phenotypic differences of asthmatic epithelium and its inflammatory responses to respiratory syncytial virus and air pollution[J]. Am J Respir Cell Mol Biol, 2011,45(5):1090-1100.
[9] Ferstl R, Akdis C A, O'Mahony L. Histamine regulation of innate and adaptive immunity[J]. Front Biosci, 2012,17:40-53.
[10] Schulzke J D, Ploeger S, Amasheh M, et al. Epithelial tight junctions in intestinal inflammation[J]. Ann N Y Acad Sci, 2009,1165:294-300.
[11] Akdis C A. Therapies for allergic inflammation: refining strategies to induce tolerance[J]. Nat Med, 2012,18(5):736-749.
[12] Suzuki T. Regulation of intestinal epithelial permeability by tight junctions[J]. Cell Mol Life Sci, 2013,70(4):631-659.
[13] Xiao C, Puddicombe S M, Field S, et al. Defective epithelial barrier function in asthma[J]. J Allergy Clin Immunol, 2011,128(3):549-556.
[14] Soyka M B, Wawrzyniak P, Eiwegger T, et al. Defective epithelial barrier in chronic rhinosinusitis: the regulation of tight junctions by IFN-gamma and IL-4[J]. J Allergy Clin Immunol, 2012,130(5):1087-1096.
[15] Kubo A, Nagao K, Amagai M. Epidermal barrier dysfunction and cutaneous sensitization in atopic diseases[J]. J Clin Invest, 2012,122(2):440-447.
[16] Akdis C A, Simons F E. Histamine receptors are hot in immunopharmacology[J]. Eur J Pharmacol, 2006,533(1-3):69-76.
[17] Simons F E, Simons K J. Histamine and H1-antihistamines: celebrating a century of progress[J]. J Allergy Clin Immunol, 2011,128(6):1139-1150.
[18] O'Mahony L, Akdis M, Akdis C A. Regulation of the immune response and inflammation by histamine and histamine receptors[J]. J Allergy Clin Immunol, 2011,128(6):1153-1162.
[19] Sawada N. Tight junction-related human diseases[J]. Pathol Int, 2013,63(1):1-12.
[20] Zabner J, Winter M, Excoffon K J, et al. Histamine alters E-cadherin cell adhesion to increase human airway epithelial permeability[J]. J Appl Physiol, 2003,95(1):394-401.
[21] Gschwandtner M, Mildner M, Mlitz V, et al. Histamine suppresses epidermal keratinocyte differentiation and impairs skin barrier function in a human skin model[J]. Allergy, 2013,68(1):37-47.
[22] Kim H M, Lee C H, Rhee C S. Histamine regulates mucin expression through H1 receptor in airway epithelial cells[J]. Acta Otolaryngol, 2012,132 Suppl 1:S37-S43.
[23] Flynn A N, Itani O A, Moninger T O, et al. Acute regulation of tight junction ion selectivity in human airway epithelia[J]. Proc Natl Acad Sci USA, 2009,106(9):3591-3596.
[24] Müller T, Myrtek D, Bayer H, et al. Functional characterization of histamine receptor subtypes in a human bronchial epithelial cell line[J]. Int J Mol Med, 2006,18(5):925-931.
[25] Matsubara M, Tamura T, Ohmori K, et al. Histamine H1 receptor antagonist blocks histamine-induced proinflammatory cytokine production through inhibition of Ca²⁺-dependent protein kinase C, Raf/MEK/ERK and IKK/I kappa B/NF-kappa B signal cascades[J]. Biochem Pharmacol, 2005,69(3):433-449.

Role of histamine in regulation of barrier function in human keratinocytes

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 2

Recommended Articles

Metrics

Comments

[1]	Li Ying, Fan Erzhong, Bo Mingyu, Wang Xiangdong, Zhang Luo. Expression of cell junctions in the tissue of chronic rhinosinusitis [J]. Journal of Capital Medical University, 2013, 34(6): 799-804.
[2]	Yang Jun;Deng Qiu;Zhou Yun;Zhu Xiaonong. Measurement of Histamine in Nasal Mucosa in Guinea Pig with Allergic Rhinitis [J]. Journal of Capital Medical University, 2006, 27(1): 109-112.