Establishment of a simple method for separation, culture and identification of mouse peripheral blood monocyte/macrophage

doi:10.3969/j.issn.1006-7795.2015.04.019

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (4): 610-613.doi: 10.3969/j.issn.1006-7795.2015.04.019

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Establishment of a simple method for separation, culture and identification of mouse peripheral blood monocyte/macrophage

Yang Lin, Tian Lei, Xie Jieshi, Li Liying

Department of Cell Biology, Municipal Laboratory for Liver Protection and Regulation of Regeneration, Capital Medical University, Beijing 100069, China

Received:2015-02-08 Online:2015-08-21 Published:2015-07-17
Supported by:
This study was supported by National Natural Science Foundation of China(81430013), Project of Construction of Innovative Teams and Teacher Career Development for Universities and Colleges Under Beijing Municipality(IDHT20150502).

Abstract

Abstract:

Objective The aim of this study is to separate, cultivate and identify mouse peripheral blood monocyte/macrophages(PBM) in L929-conditioned medium. Methods Mouse PBM was isolated by centrifugation, and cultured with L929-conditioned medium for 7 days. PBM was identified by immunofluorescence and flow cytometry(FCM). Results PBM display the immunophenotype of monocyte/macrophage and with phagocytic function. Conclusion It was a simple method for separation, culture and identification of mouse PBM in vitro.

Key words: mouse, peripheral blood macrophages, isolation and cultivation, identification

CLC Number:

Yang Lin, Tian Lei, Xie Jieshi, Li Liying. Establishment of a simple method for separation, culture and identification of mouse peripheral blood monocyte/macrophage[J]. Journal of Capital Medical University, 2015, 36(4): 610-613.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.04.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I4/610

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Establishment of a simple method for separation, culture and identification of mouse peripheral blood monocyte/macrophage

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