α-Synuclein promotes NMDA receptor internalization by upregulation of endocytosis protein Rab5B

doi:10.3969/j.issn.1006-7795.2017.06.017

Journal of Capital Medical University ›› 2017, Vol. 38 ›› Issue (6): 863-867.doi: 10.3969/j.issn.1006-7795.2017.06.017

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α-Synuclein promotes NMDA receptor internalization by upregulation of endocytosis protein Rab5B

Chen Min^1,2, Yu Wenjiao^1,3, Li Xin^1,3,4,5, Yang Weiwei^1,3,4,5, Li Xuran^1,3, Yu Shun^1,3,4,5

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing Institute of Geriatrics, Beijing 100053, China;
2. Laboratory of Neuroscience, Affliliated Hospital of Guilin Medical University, Guilin 541001, Guangxi Zhuang Automous Region, China;
3. Clinical Center for Parkinson's Disease, Capital Medical University, Beijing 100053, China;
4. Beijing Key Laboratory for Parkinson's Disease and Key Laboratory of Neurodegenerative Diseases, Ministry of Education, Beijing 100053, China;
5. National Clinical Research Center for Geriatric Disorders, Beijing 100053, China

Received:2017-10-23 Online:2017-11-21 Published:2017-12-16
Supported by:
This study was supported by National Natural Science Foundation of China (81371200, 81071014, 81401042), Beijing Municipal Administration of Hospitals' Mission Plan (SML20150803), Beijing Municipal Science & Technology Commission (Z161100005116011,Z171100000117013), Beijing Municipal Commission of Health and Family Planning (PXM2017_026283_000002), Natural Science Foundation of Guangxi (2014GXNSFAA118197).

Abstract

Abstract: Objective To investigate the effect of α-synuclein (α-Syn) on surface N-methyl-D-aspartic acid receptors (NMDARs) in cultured rat hippocampal neurons.Methods Increased intracellular α-Syn was realized by extracellular addition of recombinant human α-Syn to the culture medium or transfection of human α-Syn gene to the neurons. Western blotting analysis was applied to observe the effects of α-Syn on levels of NMDA receptor NR1 subunit and Rab5B under conditions with or without Rab5B knockdown.Results Intracellular elevation of α-Syn decreased the surface expression of NMDA NR1 and cytoplasmic levels of Rab5B; Inhibition of Rab5B expression reversed the effects of α-Syn.Conclusion The accumulation of α-Syn in hippocampal neurons can promote the internalization of surface NMDARs through an endocytic mechanism that requires participation of Rab5B.

Key words: α-synuclein, NMDA receptor, internalization, Rab5B, hippocampal neurons

CLC Number:

Q189

Chen Min, Yu Wenjiao, Li Xin, Yang Weiwei, Li Xuran, Yu Shun. α-Synuclein promotes NMDA receptor internalization by upregulation of endocytosis protein Rab5B[J]. Journal of Capital Medical University, 2017, 38(6): 863-867.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.06.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I6/863

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α-Synuclein promotes NMDA receptor internalization by upregulation of endocytosis protein Rab5B

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