Influence of Houshiheisan on the expression of axon guidance factor and Rho GTPases

doi:10.3969/j.issn.1006-7795.2017.06.022

Abstract

Abstract: Objective To investigate the influence of Houshiheisan on the expression of axon guidance factor Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA in cerebral ischemia injury rats.Methods The permanent middle cerebral artery occlusion (pMCAO) model was made by the intraluminal suture method. Rats were randomly divided into sham group, model group, low dose of Houshiheisan group, middle dose of Houshiheisan group and Ginaton group.Western blotting and RT-PCR were employed to detect the expression of Netrin-1, DCC, Rac1, Cdc42 and RhoA in ischemic cerebral cortex of rats 7 days after operation. Results Compared with the model group,in the middle dose of Houshiheisan group and Ginaton groups, the expression of Netrin-1 protein was elevated significantly(P<0.01),middle dose of Houshiheisan group, the expression of Netrin-1 in mRNA was significantly increased(P<0.05); in the low dose of Houshiheisan, middle dose of Houshiheisan and Ginaton groups, the expression of DCC, Rac1 and Cdc42 protein and mRNA (P<0.05, P<0.01)was significantly increased; but the expression of RhoA protein and mRNA was decreased significantly (P<0.01).Conclusion The findings demonstrated that Houshiheisan could accelerate the recovery of neurological impairment after celebral ischemia by regulating the expression of axon guidance factors like Netrin-1/DCC and molecular switch Rac1/Cdc42/RhoA.

Key words: cerebral ischemia, Houshiheisan, Netrin-1, DCC, Rac1, Cdc42, RhoA

CLC Number:

R289.5

Lu Yue, Liu Jianhong, Zhao Hui, Chang Jiahui, Xiang Yangyang, Zhang Qiuxia. Influence of Houshiheisan on the expression of axon guidance factor and Rho GTPases[J]. Journal of Capital Medical University, 2017, 38(6): 891-896.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.06.022

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I6/891

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