Effect of Qingdu decoction on the protein expressions of Bax, Bcl-2 in rats with severe liver injury

doi:10.3969/j.issn.1006-7795.2018.01.015

Journal of Capital Medical University ›› 2018, Vol. 39 ›› Issue (1): 79-83.doi: 10.3969/j.issn.1006-7795.2018.01.015

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Effect of Qingdu decoction on the protein expressions of Bax, Bcl-2 in rats with severe liver injury

Jin Shuang, Gao Lianyin, Zhao Mengpei, Che Niancong, Zhang Qiuyun, Du Yuqiong

Department of Traditional Chinese Medicine Clinical Basis, Traditional Chinese Medicine Academy, Capital Medical University, Beijing Key Laboratory of Traditional Chinese Medicine Collateral Disease Research, Beijing 100069, China

Received:2017-09-04 Online:2018-01-21 Published:2018-01-27
Supported by:
This study was supported by Natural Science Foundation of Beijing(7142023), Capital of Traditional Chinese Medicine Special Research of Beijing Municipal Education Commission(16ZY01).

Abstract

Abstract: Objective To observe the effects of Qingdu decoction on the protein expressions of Bax,Bcl-2 and the apoptosis of liver cells in rats with severe liver injury and explore the mechanism of Qingdu decoction on treating hepatocyte apoptosis of the chronic severe hepatitis.Methods The severe liver injury rat model was induced by thioacetamide (TAA),and treated with Qingdu decoction. And then the hepatocyte apoptotic index(AI) was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling(TUNEL) assay and the protein level of Bax,Bcl-2 in hepatic tissue was detected by Western blotting.Results The AI in model group was significantly increased when compared to that of in normal group (P<0.05).Compared to the model group,the AI was decreased significantly in each group of Qingdu decoction (P<0.01 or P<0.05).To the severe liver injury rat model, Qingdu decoction can cause the inhibition of the protein expressions of Bax (P<0.01),and dramatically increase the protein expressions of Bcl-2(P<0.01)。Conclusion Qingdu decoction can regulate the protein expression level of Bax and Bcl-2,decrease AI, alleviate the degree of liver injury and apoptosis.

Key words: Qingdu decoction, chronic severe hepatitis, apoptotic index (AI), Bax, Bcl-2

CLC Number:

R289
R575.1

Jin Shuang, Gao Lianyin, Zhao Mengpei, Che Niancong, Zhang Qiuyun, Du Yuqiong. Effect of Qingdu decoction on the protein expressions of Bax, Bcl-2 in rats with severe liver injury[J]. Journal of Capital Medical University, 2018, 39(1): 79-83.

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Effect of Qingdu decoction on the protein expressions of Bax, Bcl-2 in rats with severe liver injury

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