Effect of angiotensin-converting enzyme 2 and angiotensin(1-7) on lipogenesis in white adipose tissue of mice

doi:10.3969/j.issn.1006-7795.2018.04.009

Abstract

Abstract: Objective To identity the effect of angiotensin-converting enzyme 2(ACE2) and angiotensin (1-7) (Ang(1-7)) on lipogenesis in white adipose tissue of mice. Methods Male ACE2-knockout (ACE2KO) mice and C57BL/6(wild-type, WT) mice were chosen. The body weight, body fat percentage, total serum cholesterol, and triglycerides were measured. Hematoxylin and eosin staining was employed to observe the morphology changes of epididymal adipocyte. The protein expression of lipogenesis related genes including acetyl-CoA carboxylase a (ACCa), fatty acid synthetase(FAS) and adipocyte fatty acid binding protein (aP2) in epididymal adipose tissue was assayed by Western blotting. Male db/db mice were randomly divided into three groups. Mice were treated with normal saline (NS), A779[D-Ala7-Ang(1-7),Ang(1-7) antagonist], and Ang(1-7)+A779[Ang(1-7) antagonist] for 28 days using the micro-osmotic pump. The body weight was measured, and the protein expression of lipogenesis related genes in epididymal adipose tissue was assayed by Western blotting. Results The body fat percentage and serum triglycerides in ACE2KO mice were higher than those in WT mice. The protein expression of ACCa and FAS was increased in ACE2KO mice epididymal adipose tissue than control mice. In db/db mice, Ang(1-7) treatment downregulated the protein expression of ACCa and FAS in epididymal adipose tissue, A779 inhibited the effect. Conclusion ACE2/Ang (1-7) may inhibit lipogenesis in white adipose tissue by reduction of lipogenesis-related genes.

Key words: renin-angiotensin system, angiotensin-converting enzyme 2, angiotensin(1-7), lipogenesis

CLC Number:

R781.6

Ma Chifa, Shi Tingting, Liu Jingyi, Song Lini, Feng Jianping, Yuan Mingxia, Yang Jinkui. Effect of angiotensin-converting enzyme 2 and angiotensin(1-7) on lipogenesis in white adipose tissue of mice[J]. Journal of Capital Medical University, 2018, 39(4): 527-531.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.04.009

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I4/527

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