Prevalent HPV 16 and 18 infection and vaginal microbiota in initial screening women

doi:10.3969/j.issn.1006-7795.2018.06.011

Abstract

Abstract: Objective To evaluate the association between human papillomavirus (HPV) 16, 18 and the vaginal microbiome. Methods Totally 78 women who underwent initial HPV screening in Beijing Chaoyang Hospital between January and June in 2016 were recruited into this study. Vaginal bacterial composition was characterized by deep sequencing of barcoded 16S rRNA gene fragments (V4) on Illumina MiSeq and HPV was identified using the Shanghai ZJ Bio-tech Array © HPV genotyping test. The exact Logistic regression models were applied to evaluate the association between community state types (CSTs) of vaginal microbiota and HR-HPV infection, weighted UniFrac distances were used to compare the vaginal microbiota of individuals with prevalent HR-HPV to those without prevalent HR-HPV infection, and the Linear Discriminant Analysis effect size (LEfSe) algorithm was applied to characterize bacteria associated with prevalent HR-HPV infection. Results Three CSTs were observed:CST IV with a low relative abundance of low Lactobacillus spp. in 65.4% of participants; CST Ⅲ(dominated by L. iners) in 32.1%; CST V(dominated by L. jensenii) in 2.6%; LEfSe analysis suggested an association between the prevalent HPV-16 infection and the decreased abundance of Lactobacillus spp. with increased abundance of anaerobes particularly of the genera Streptococcus agalactiae which were only found in HPV-16 positive women (P<0.05). A predominance of Lactobacillus spp. was still present in HPV-18 positive women with depletion of strictly anaerobic species. ConclusionHPV-16 infection was associated with the composition and abundance of vaginal microflora and Streptococcus agalactiae can be the specific bacterial taxa of HPV-16 positive women, Further studies were needed to confirm these finding.

Key words: human papilloma virus, vaginal microbiota, cervical intraepithelial neoplasias, Illumina MiSeq

CLC Number:

R737.33

Luo Mei, He Xin, Liu Jun, Zhang Xuefang, Zhang Jianxin, Wang Qiuxi, Wang Shuzhen, Diao Xiaoli, Qu Jiuxin. Prevalent HPV 16 and 18 infection and vaginal microbiota in initial screening women[J]. Journal of Capital Medical University, 2018, 39(6): 849-857.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.06.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I6/849

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