Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats

doi:10.3969/j.issn.1006-7795.2021.04.014

Abstract

Abstract: Objective To observe the effects of different hypobaric and hypoxic stimulation time on the average pulmonary artery pressure, the expression levels of the vascular endothelial growth factor (VEGF), the platelet derived growth factor(PDGF) and the fibroblast growth factor(FGF) in serum of rats. Methods Thirty two male SD rats were divided into four groups: group A was fed for 21 days at normal altitude, group B was kept in hypobaric hypoxia environment for 21 days (simulated altitude of 5 000 meters in hypobaric hypoxia chamber), group C was 6 hours a day in hypobaric hypoxia environment for 21 days, group D was interrupted for 24 hours (the next day)in hypobaric hypoxia environment for 21 days, and the factors of light, diet and water were the same. The pulmonary artery pressure of each group was measured by right cardiac catheterization, the lung tissue of each group was observed by HE staining, and the expression of VEGF, PDGF and FGF in serum of each group was measured by ELISA. Results ① the average pulmonary artery pressure in group B, C and D was higher than that in group A (P<0.05), the most significant increase was (27.4±1.2) mmHg (1 mmHg=0.133 kPa) in group B, followed by (22.1±1.1) mmHg in Group D. ② In group B, C and D, lymphocyte infiltration was found around the pulmonary vessels. In group B and D, inflammatory cells and fibrous tissue caused local consolidation were found in the lung tissue, and local alveolar hyperinflation was also found, presenting as emphysema. ③ The expression of VEGF, PDGF and FGF in group B, C and D were significantly higher than that in group A (P<0.05). Conclusion Intermittent hypobaric hypoxia stimulation can increase pulmonary arterial pressure in rats, but the average pulmonary artery pressure of rats with intermittent hypobaric hypoxia for 6 hours or 24 hours did not reach 25 mmHg during 21 days; Intermittent hypobaric hypoxia leads to the increase of inflammatory cell infiltration and the destruction of alveolar wall structure, which leads to pulmonary consolidation and emphysema; The expression of VEGF, PDGF, FGF was significantly increased.

Key words: hypobaric hypoxia, pulmonary artery pressure, lung tissue

CLC Number:

Deng Hui, Wang Yong, Chen Xiaolan, Qin Chong, Pan Lei. Effects of hypobaric hypoxia stimulation at different times on pulmonary arterial pressure and lung tissue in rats[J]. Journal of Capital Medical University, 2021, 42(4): 596-600.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.04.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I4/596

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