Abstract: Objective To study the expressions of somatostatin receptor subtypes in human activated hepatic stellate cells(LX-2）.Its clinical significances was also surveyed.Methods To detect the expressions of SSTR2/5 of LX-2 which is in the logarithmic growth cycle using immunocytochemistry staining method: LX-2 were routinely cultured on small glass coverslip in six well cluster dishes at a density of 8×10⁴ cells/well.The cells were divided into 5 subgroups: compound 861 0.5 g/L,compound 861 1.0 g/L,octreotide 1 mg/L and control group,PNAC-1 was a positive control cell line.Meantime,expressions of SSTR1-5 mRNA were observed using RT-PCR method.Results The expressions of SSTR 2,SSTR5 in LX-2 were positive after immunocyto-chemistry staining.The mainly staining site is cellular membrane and cellular plasm.The strong expressions in compound 861-treated group were also observed.The five kinds of subtypes of SSTR in LX-2 were proven to be positive via the detection of RT-PCR.To compared the level of the subtypes,SSTR1,4>SSTR2,5>SSTR3 was observed.Conclusion LX-2 expresses five kinds of somatostatin receptors subtype.It is suggested that the activated hepatic stellate cells is target of SST and its analogs.

Key words: hepatic stellate cell, somatostatin receptor subtype, compound 861, octreotide