Effects of Proliferation Inhibition and Apoptosis Induced by COX-2 Selective Inhibitor on Esophageal Squamous Carcinoma Cells

Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (4): 438-440.

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Effects of Proliferation Inhibition and Apoptosis Induced by COX-2 Selective Inhibitor on Esophageal Squamous Carcinoma Cells

Li Peng¹, Zhang Shutian¹, Yu Zhonglin¹, Liu Xuan¹, Zhang Li¹, Zhou Qiaozhi¹, Lin Jin¹, Hu Yaojun¹, Xu Caimin²

1. Department of Digestive Diseases, Beijing Friendship Hospital, Capital University of Medical Sciences;2. National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College

Received:2006-06-16 Revised:1900-01-01 Online:2006-08-24 Published:2006-08-24

Abstract

Abstract:

Objective To investigate the effects of nimesulide(a non-selective cyclooxygenase-2 inhibitor） on cell proliferation inhibition and apoptosis in esophageal squamous cell carcinoma(ESCC） cell line.Methods EC109 cells were incubated with nimesulide.Cell proliferation was assessed by MTT assay.Cell apoptosis induction were determined by fluorescence staining flow cytometry.PGE₂ production was measured by ELISA assay to determine the effect of nimesulide on cyclooxygenase activity.Results Nimesulide inhibited cell growth in a concentration and time dependent manner.Apoptosis was induced by Nimesulide dose dependently.This was accompanied with a decrease of PGE₂ production.Conclusion Nimesulide could inhibit ESCC cell proliferation and induce apoptosis,which is probably through cyclooxygenase-2 inhibition.Cyclooxygenase-2 inhibitor may be a promising therapeutic agent for human ESCC.

Key words: esophageal squamous cell carcinoma, cyclooxygenase-2, growth inhibition, apoptosis

CLC Number:

R735.1

Li Peng;Zhang Shutian;Yu Zhonglin;Liu Xuan;Zhang Li;Zhou Qiaozhi;Lin Jin;Hu Yaojun;Xu Caimin. Effects of Proliferation Inhibition and Apoptosis Induced by COX-2 Selective Inhibitor on Esophageal Squamous Carcinoma Cells[J]. Journal of Capital Medical University, 2006, 27(4): 438-440.

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Effects of Proliferation Inhibition and Apoptosis Induced by COX-2 Selective Inhibitor on Esophageal Squamous Carcinoma Cells

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