Abstract: Objective To establish a method for quantifying the expression level of Per1 in mouse striatum.Methods Total RNA isolated from mouse striatum was subjected to reverse transcription and amplification with Per1 specific primers.SYBR green I was used for real-time PCR detection.Results Parameters in amplification and detection were determined.The quantification system was highly specific and the amplification efficiency was more than 90%.Expression of Per1 showed a daily oscillation.Conclusion This system could used for quantifying mRNA level of Per1 with high sensitivity and broad linearity.The time-dependent expression of Per1 in striatum suggested that the interaction between Substantial Nigra and striatum may oscillate in a daily rhythmic base.

Key words: Per1, real-time RT-PCR, SYBR green I, striatum