Abstract:

Objective To amplify the gene sequence of CD14, analyze the structure and bio-information of the cloned genes. Methods Human soluble CD14(sCD14) gene cDNA fragment was amplified from total RNA extracted from U₉₃₇ cells by RT-PCR. The bio-information of cloned CD14 gene and translated protein was then analyzed, DNA and amino sequence, protein prosite and stabilization, alpha-helix/Deleage and Roux were identified. Results The amplified nucleotide sequence fragment consistent completely with the published human CD14 sequence. Moreover, we knew clearly gene structure and bio-information parameter of human soluble CD14 according the sequence. Conclusion The co-index of the gene of CD14 is achieved successfully. These results make it possible to identify Human recombinant CD14 gene.

Key words: CD14, gene cloning, bio-information