Chemiluminescence Immunoassay for Detection of SARS-CoV Protein Antigen

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (3): 293-295.

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Chemiluminescence Immunoassay for Detection of SARS-CoV Protein Antigen

Zhen Yaping¹, Liu Hong², Qi Na¹, Shan Ruoming¹, Wang Qiaoqiao³, Wu Xiaodong³

1. Clinical Inspect Center, Capital University of Medical Sciences;2. People's Hospital, Peking University;3. Beijing Yuande Biomedical Engineering Co.LTD

Received:2004-09-21 Revised:1900-01-01 Online:2005-06-24 Published:2005-06-24

Abstract

Abstract:

Objective To establish a laboratory method for rapid, simple and sensitive diagnosis of SARS. Methods Culture supernote of 34 viruses strains and sera of SARS patients were assayed by CLIA. Results The experiments indicated high specificity and sensitivity to viruses strains, reactivity with 6 SARS strains and not cross-reactivity with 11 others strains, the detectability was on more then 6.3×10¹ PFU/mL of SARS-CoV strain. For the 63 SARS patients, positive rate was found to be 100% during the disease day of 6～10 and for the 1 066 normal sample, negative rate was 99.35%. Conclusion The sensitivity and specificity of CLIA for SARS-CoV detection is higher satisfying. It is rapid and simple. It might be used to earliest diagnosis of SARS-CoV infection.

Key words: chemiluminescence, immunosaasy, SARS, N protein

CLC Number:

R392.33

Zhen Yaping;Liu Hong;Qi Na;Shan Ruoming;Wang Qiaoqiao;Wu Xiaodong. Chemiluminescence Immunoassay for Detection of SARS-CoV Protein Antigen[J]. Journal of Capital Medical University, 2005, 26(3): 293-295.

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Chemiluminescence Immunoassay for Detection of SARS-CoV Protein Antigen

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