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Separation, purification and identification of mouse bone marrow neutrophils Yang Lin, Tian Lei, Zhou Xuan, Yang Le, Li Liying Journal of Capital Medical University    2018, 39 (5): 699-703.   DOI: 10.3969/j.issn.1006-7795.2018.05.014 Abstract （570）      PDF（pc） （2781KB）（846）       Knowledge map    Save Objective For separation, purification and identification of mouse neutrophils from bone marrow. Methods Mouse bone marrow neutrophils were separated by density gradient centrifugation. Immunofluorescence and High Content Screening were used to detect the expression of Ly6G. Phorbol 12-myristate 13-acetate (PMA) was used to induce the expression of myeloperoxidase(MPO) released by neutrophils by immunofluorescent staining. Results After the mouse bone marrow cells were purified with Histopaque©-1077, Histopaque©-1119, the cells expressed Ly6G. High Content Screening was used to detect the expression of MPO, as demonstrated by the increased expression of MPO (3.26±0.22) stimulated by 100 nmol/L PMA for 2 hours. Conclusion It was a simple method for separation, purification and identification of mouse neutrophils from bone marrow. Reference | Related Articles | Metrics

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Clinical application of electromagnetic navigation bronchoscopy in the diagnosis and treatment of peripheral lung lesions Tan Xiaogang, Zhang Yi Journal of Capital Medical University    2022, 43 (4): 570-575.   DOI: 10.3969/j.issn.1006-7795.2022.04.010 Abstract （135）      PDF（pc） （1007KB）（91）       Knowledge map    Save As low-dose computed tomography(CT) is applied more and more in high-risk populations for lung cancer screening, the detectable rate of peripheral pulmonary lesions (PPLs) also increases. Electromagnetic navigation bronchoscopy (ENB) is a tool that can diagnose, locate and treat PPLs. Because of its higher safety and reliability, the application of ENB may change the methods of diagnosis and treatment for lung diseases in the future so as to alleviate and even cure lung cancer, and path a way for the treatment of lung cancer. Reference | Related Articles | Metrics

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A preliminary study on the therapeutic efficacy of Duloxetine in the treatment of depersonalization-derealization disorder Zheng Sisi, Song Mingkang, Song Nan, Zhu Hong, Jia Hongxiao Journal of Capital Medical University    2023, 44 (4): 578-582.   DOI: 10.3969/j.issn.1006-7795.2023.04.012 Abstract （171）      PDF（pc） （2217KB）（156）       Knowledge map    Save Objective  To explore the efficacy and influencing factors of Duloxetine in the treatment of depersonalization-derealization disorder (DPD). Methods  The data were collected from January 2020 to December 2022 for DPD patients treated with Duloxetine at Beijing Anding Hospital,  Capital Medical University. Survival analysis was used to analyze treatment efficacy and influencing factors. Results  A total of 51 patients were included in this study, and 64.7% of the patients showed treatment efficacy. The median effective time was 118 (87, 643) days, and there was a trend of rapid improvement in emotional numbness symptoms. Education level was identified as a significant influencing factor for duloxetine treatment, with HR=0.81 (0.70, 0.95), P=0.008. Conclusions  Duloxetine treatment for DPD, particularly for the patients with emotional numbness as the main complaint, has potential benefits and provides guidance for clinical treatment of DPD. Reference | Related Articles | Metrics

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Journal of Capital Medical University    2003, 24 (3): 350-352.   Abstract （402）      PDF（pc） （375KB）（256）       Knowledge map    Save Related Articles | Metrics

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Persistence and clearance high risk human papillomavirus types in the population of primary infection: a follow-up study Xu Shasha, He Xin, Liu Yingqiao, Liu Jun, Wang Shenzhen, Wang Yue, Diao Xiaoli, Qu Jiuxin Journal of Capital Medical University    2015, 36 (2): 212-218.   DOI: 10.3969/j.issn.1006-7795.2015.02.010 Abstract （382）      PDF（pc） （2545KB）（484）       Knowledge map    Save Objective To investigate the persistence and clearance data regarding the 13 types of high risk human papillomavirus(HR-HPV) in the population of primary infection. Methods A total of 969 cases of HR-HPV positive patients were followed up between January 1, 2013 and December 31, 2013. The epidemiological characteristics of HR-HPV persistent infection were analyzed. The age, viral genotyping, HR-HPV multiple genotypes and initial viral load were evaluated. Statistical analyses were performed using SPSS software for Windows (version 18.0). The Kaplan-Meier survival function was used for calculating the time taken for the clearance of HR-HPV. The role of a group of independent variables concerning the time taken until clearance was evaluated using a Cox proportional-hazards regression model. P<0.05 was considered statistically significant. Results The average time of viral clearance was 9.52 months for all of 969 cases(9.09-9.95 months 95% CI). Older women had longer clearance time than the younger women groups(χ2=7.63, P=0.45). It was found that HPV-56, 35, 58, 52 and 18 had the lower probability of being cleared, when that HPV-45, 51 and 68 had the shorter time to be cleared. It was also found that multiple viral infection had the lower probability of being cleared than the single ones(χ2=18.67, P=0.000). Lower viral loads had better clearance compared to those having higher viral loads(χ2=18.68, P=0.000)). It was shown that age (regression coefficient=-0.082, P=0.046)and single or multiple HR-HPV infection (regression coefficient=-0.231, P=0.037) were associated with time to clearance in the present sample, while the lower or higher viral loads (regression coefficient=-0.185, P=0.108) was not associated with time to clearance in the Cox proportional-hazards regression model. Conclusion HPV infection clearance in this study was related to factors such as age, infection type, viral load and the multiple or single viral infections. Reference | Related Articles | Metrics Cited: Baidu(1)

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Establishment of Animal Model of Human Cervical Cancer in BALB/c Nude Mice by Tumor Tissue Xenograft Zhang Kaiju;Zhao Yanzhong;Shang Hongwei;Kong Lu;Zhang Yuxiang Journal of Capital Medical University    2008, 29 (5): 593-596.   Abstract （1508）      PDF（pc） （576KB）（1287）       Knowledge map    Save Objective To establish the human cervical cancer animal model through abdominal subcutaneous transplantation in BALB/c nude mice and investigate the rate of tumor-forming and the biological features of the tumor.Methods Human cervical cancer tissue derived from surgical specimens were transplanted into the subcutis of nude mice.The rate of tumor transplantation,generic characteristic,tumor growth of bearing cancer mice were observed every day.On day 85 the tumor-bearing mice were killed and tumor masses were collected for analysis of histological features.Genomic DNAof tumor tissue,peripheral blood,liver,spleen,kidney,omentum,and uterus annex of tumor-bearing BALB/c nude mice were extracted,and then using all organs' genomic DNAas templates,we detected the HPV DNAexpression with polymerase chain reaction(PCR).Results In this experiment,the delitescence period of tumor-forming were 30～35 days.On day 42 the tumor tubercles were visible and grew fast day by day.The average tumor-take rate was 90.5%,and the mean tumor surface areas was(50.0±5.1)mm2 on day 80.The transplanted tumors grew well by local invasion.Metastasis was not found.The tumors were analyzed by HEstaining,and the histological appearance of the tumors before and after transplantation were very similar.The positive rate of HPV DNAin the tumor tissue was 100%.The appearance of HPV DNAbefore and after transplantation was the same.The PCRresults definitely displayed the subtype of high-risk human papillomavirus,which suggested cervical cancer patient's HPV16 infection,not HPV18 infection.But HPV DNAwas not detectable in peripheral blood,the spleen,the liver,the kidney,the omentum,and the uterus annex of tumor-bearing BALB/c nude mice.Conclusion Human cervical cancer model in nude mice has been successfully established which simulates the biological features of human cervical cancer.The animal model of human cervical cancer in BALB/c nude mice exhibit a high rate of tumor transplantation,and which may provide a convenient and ideal tool for the research of the mechanism and therapy of human cervical cancer. Related Articles | Metrics Cited: Baidu(4)

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Journal of Capital Medical University    2002, 23 (3): 277-280.   Abstract （508）      PDF（pc） （436KB）（2105）       Knowledge map    Save Related Articles | Metrics

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Comparison between Different Methods to Extract Mitochondria and Its Effects on Mitochondria Content and Activity WU Yuan;DONG Ling-yue;AN Wei;AN Yun-qing Journal of Capital Medical University    2010, 31 (2): 241-244.   Abstract （1537）      PDF（pc） （419KB）（2642）       Knowledge map    Save Objective To compare different methods for extracting mitochondria and its effects on mitochondria content and their activity. Methods Pulilai kit, sucrose gradient centrifugation and Pierce mitochondria extraction kit were used to extract mitochondria and quantify their concentration, activity and contamination. Results Pulilai kit yielded higher concentration of mitochondria proteins than two other methods(P<0.05) when the same amount of cells were used. Western blotting was used to evaluate the amount of cytosolic protein contaminant and mitochondria protein cytochrome c from mitochondria fraction. The result suggests that using Pulilai kit can yield lower concentration cytosolic protein contaminant and higher concentration of cytochrome c from mitochondria fraction than the other two methods. At the same time, ATP content of mitochondria extracted by the method of Pulilai kit was the greatest. Conclusion Pulilai kit is better than sucrose gradient centrifugation and Pierce mitochondria extraction kit for mitochondria extraction. Related Articles | Metrics Cited: Baidu(8)

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Journal of Capital Medical University    1980, 1 (4): 333-340.   Abstract （670）      PDF（pc） （3305KB）（1633）       Knowledge map    Save Related Articles | Metrics

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Research progress of cathepsin in the mechanism of diabetes and diabetic nephropathy Li Mingjia, Zhao Miaomiao , Yang Jinkui Journal of Capital Medical University    2023, 44 (3): 413-419.   DOI: 10.3969/j.issn.1006-7795.2023.03.009 Abstract （137）      PDF（pc） （1647KB）（116）       Knowledge map    Save Cathepsin is one of the most important proteolytic enzymes in mammals, which exists both intracellularly and extracellularly. It participates in various physiological and pathological processes in vivo such as regulating autophagy, cell apoptosis, maintaining extracellular matrix homeostasis, antigen presentation, viral infection and inflammation. In recent years, cathepsin B (CTSB), cathepsin D (CTSD), cathepsin L (CTSL), and cathepsin S (CTSS) have been considered to be the main players in metabolic diseases. They are involved in the pathogenesis and progression of  mellitus (DM） and diabetic nephropathy (DN) and may become potential new targets for prediction and treatment. This article reviews the role and mechanism of CTSB, CTSD, CTSL, and CTSS in DM and DN. Reference | Related Articles | Metrics

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The Existent Form of Mercury and Arsenium in Angong Niuhuang Wan and the Absorption and Excreting in Rats Li Jun;Yuan Zhuobin;Han Shubo;Zhu Min Journal of Capital Medical University    2006, 27 (5): 647-651.   Abstract （679）      PDF（pc） （489KB）（450）       Knowledge map    Save Objective To detect the existent form of mercury and arsenium in Angong Niuhuang Wan(ANW) and the absorption and excreting in rats.Methods The existent form of mercury was detected with high performance capillary electrophoresis.Mercury and arsenium of vermilion and realgar in artificial gastric and intestinal juice,and mercury and arsenium excreted out of rats fed with mercuric sulfide,arsenic disulfide and ANW were respectively tested with cold vapour-atomic absorption spectrometry and hydrogen arsenide based method.Results Mercury scutellarin was detected.Mercury of vermilion in artificial gastric and intestinal juice respectively was 0.003 05% and 0.000 04%.Arsenium in those Mercury and arsenium of vermilion and realgar were respectively was 0.033 75% and(0.000 61%).24 h later,more than 90.195 0% mercury was excreted out of rats fed with mercuric sulfide;26.437 9% arsenium was excreted out of rats fed with arsenic disulfide;82.830 9% mercury and 24.442 0% arsenium were excreted out of rats fed with ANW.The difference of excreted mercury and arsenium is not significant between rats fed with ANW and rats fed with mercuric sulfide and arsenic disulfide.Conclusion The absorption and excretion of mercury and arsenium from ANW,mercuric sulfide and arsenic disulfide in rats are analogical. Related Articles | Metrics

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Whole brain electroencephalogram difference research: effects of unilaterally closed and open eye WANG Kun;CUI Wei-qi;LI Guo-zhen;CHEN Andrew CN Journal of Capital Medical University    2012, 33 (2): 205-208.   DOI: 10.3969/j.issn.1006-7795.2012.02.014 Abstract （931）      PDF（pc） （2148KB）（1173）       Knowledge map    Save Objective Electroencephalogram(EEG) is a modern examination method, which amplifies tiny bioelectrical signals of brain itself. This study focused on the differences of seven EEG spectral regional field powers: Delta(0.5~3.5 Hz), Theta(4~7 Hz), Alpha-1~(7.5~9.5 Hz), Alpha-2(10~12 Hz), Beta-1(13~23 Hz), Beta-2(24~34 Hz) and Gamma(35~45 Hz)) when the unilateral eye(right and left eye) is open and closed. Methods Fifteen healthy subjects(all were men; age: 19~27 years), in the resting state, in a quiet and comfortable room, who were asked to close and open unilateral eye(left-closed/open, right-closed/open) in counter-balanced order stimulation, while respective EEG was recorded and analyzed. Results In the resting state, regional EEG field powers of mid-frequency band Alpha-1 and Alpha-2 field powers were obviously higher in eyes-closed than in eyes-open state(Alpha-1, P=0.038; Alpha-2, P=0.02). Compared unilateral eye-closed and eye-open, there were no significant differences in alpha bands or other bands. Conclusion Alpha band power was significantly higher when subjects closed eyes than opened their eyes, however, when we compared unilateral eye-closed and eye-open differences of seven bands, significant differences was uncovered. We conclude that bilateral EEG powers are balanced via corpus callosum in persistent 2 min unilateral stimulation. Related Articles | Metrics Cited: Baidu(1)

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Application research hotspot of traditional Chinese medicine informatics Gao Man, Li Haiyan Journal of Capital Medical University    2022, 43 (4): 592-599.   DOI: 10.3969/j.issn.1006-7795.2022.04.013 Abstract （120）      PDF（pc） （1014KB）（89）       Knowledge map    Save This paper summarized the popular applications of traditional Chinese medicine(TCM) informatics research to provide useful references for researchers, which include TCM clinical data mining and utilization, TCM intelligent diagnosis and treatment equipment research, Chinese medicine information research, discipline knowledge service based on TCM ancient and modern literature information, and TCM evaluation research based on big data. Reference | Related Articles | Metrics

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Progresses on the effect of estrogen on innate immune cells Li Ziyi, Wang Xi, Liang Pu Journal of Capital Medical University    2023, 44 (2): 351-357.   DOI: 10.3969/j.issn.1006-7795.2023.02.025 Abstract （110）      PDF（pc） （480KB）（94）       Knowledge map    Save The immune system has gender dimorphism. Females mount stronger innate immunity and adaptive immune response  than males, and female sensitivity to multiple pathogenic infections and malignant tumors is lower. Meanwhile, women are more likely to develop autoimmune diseases. One of the important factors responsible for these sex differences, estrogen, exerts a wide range of biological effects in the human body. Currently, estrogen has been reported to regulate the differentiation, maturation, longevity, and effector function of innate immune cells, including neutrophils, monocytes and macrophages, natural killer cells, and dendritic cells. This review discusses in detail how estrogen regulates the normal physiological function of immune cells and its different roles in various disease states. Reference | Related Articles | Metrics

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Studies on Treatment to InfrapateIlar Fat Pad Syndrome in 22 Patients Liu Deyun;Li Liping Journal of Capital Medical University    2008, 29 (6): 712-713.   Abstract （715）      PDF（pc） （116KB）（675）       Knowledge map    Save Objective To provide an effective the treatment method for hyperplasia and hypertrophy with clinical symptoms induced by injury of infrapatellar fat pad.Methods Atotal of 22 patients with infrapatellar fat pad syndrome were received in our hospital between the years 1996 and 2007,12 of them underwent surgery,10 of them underwent outpatient treatment.Results All patients received follow up 2～8 years after surgery.It disappeared that the symptoms and signs caused by hypertrophy of fat pad disappeared in the postoperative patients,while they aggravated in un-operative patients.Conclusion Once the hypertrophy and hyperplasia of the infrapatellar fat pad occurs,surgery is the best way to resect the whole fat pad,and it is hard to cure by the use of drugs. Related Articles | Metrics

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Protective Effect of Abelmosehus Manihot on Rats Renal Tubular with Nephrotic Syndrome Yin Lianfang;Liu Lu;Gong Yuxiang;Pu Jiakang Journal of Capital Medical University    2000, 21 (3): 209-211.   Abstract （595）      PDF（pc） （309KB）（662）       Knowledge map    Save To investigate the protective effects of Abelmosehus manihot (Am) on rat renal tubular with nephrotic syndrome, the nephrotic rat model was made by tail vein injection of adriamycin 5mg/kg. Am was given and changes of urinary type Ⅲ collagen(C-Ⅲ),N-acetyl-β-D-glucosa minidase(NAG)and β2 microglobulin(β2-MG) were observed. It was shown that the excretion of urinary C Ⅲ,NAG and β2-MG in nephrotic rats were significantly increased as compared with those of the normal control group (P< 0.01). In the group treated with Am, the excretion of urinary C-Ⅲ,NAG and β2-MG did not increase obviously . Am exerted strong protective effects on rats renal tubular damage with adriamycin induced nephrotic syndrome. Related Articles | Metrics

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Clinical and Pathological Analysis of 23 Cases of Necrotizing Lymphadenitis Su Wenjuan;Chen Bingliang;Xue Fengwen;Hu Hongying;Li Xiaoying Journal of Capital Medical University    1998, 19 (2): 130-132.   Abstract （539）      PDF（pc） （393KB）（287）       Knowledge map    Save Clinical manifestation and pathological morphology of 23 cases of necrotizing lymphadenitis were reported. This disease occurs in young female more frequently. Its chief clinical manifestations are enlargement of lymph node in neck and fever. Its histological change is complicated. The differential diagnosis must be made between necrotizing lymphadenitis and other lymphadenitis or malignant lymphoma. Biopsy of lymph node is an important diagnostic mean. The patient recovers after a self limited clinical course. Antibiotics aren′t effective regimen. Related Articles | Metrics

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Antioxidant activity of the crude extract and the active ingredient extracted from Radix Paeoniae Alba XIA Ying, YIN Zhishuang, SHI Chen, WANG Qiao, SONG Xueying Journal of Capital Medical University    2013, 34 (1): 120-125.   DOI: 10.3969/j.issn.1006-7795.2013.01.023 Abstract （1147）      PDF（pc） （3279KB）（966）       Knowledge map    Save Objective To study antioxidant activity of the crude extract and the active ingredient (pentagalloylglucose, PGG) extracted from Radix Paeoniae Alba.Methods The extracts obtained from Radix Paeoniae Alba by ultrasonic extraction with 50% of ethanol as extracting solvent. High-speed counter-current chromatography (HSCCC) was used for separation and purification of PGG in the crude extract from Radix Paeoniae Alba. PGG was analyzed and identified by HPLC and ESI-MS methods. The antioxidant activity of the crude extract and PGG were evaluated by1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and ferric reducing-antioxidant power(FRAP) assay and vitamin C (Vc) was used as a positive control. Results The purity of PGG was 95.7%, which was separated and purified in the crude extract from Radix Paeoniae Alba. DPPH assay showed that both of crude extract and PGG have strong scavenging effect on DPPH. The residual rates of the IC50 were PGGcrude extract>Vc. FRAP assay showed that the PGG was the strongest antioxidant in comparison with the crude extract and Vc especially at lower concentrations (eg, 10 μg/mL). As the concentration increased, the FRAP values of each sample increased, and antioxidant abilities of samples also increased simultaneously. However, FRAP values of Vc increased more significantly. At the concentration of 100 μg/mL, FRAP value of Vc was 420.8 μmol/L that was very close to the FRAP value of crude extract at the same concentration, but still below FRAP value of PGG of 567.1 μmol/L.Conclusion It was shown that PGG, an ingredient extracted from Chinese medicine Radix Paeoniae Alba, has antioxidant activity. The antioxidant effect of PGG was much higher than that of the positive control Vc. PGG may become an effective anti-oxidant with a wide application for clinical treatment of diseases. Reference | Related Articles | Metrics Cited: Baidu(8)

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Journal of Capital Medical University    2001, 22 (1): 91-93.   Abstract （360）      PDF（pc） （255KB）（408）       Knowledge map    Save Related Articles | Metrics

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Correlation between the counting results of Durham pollen sampler and automatically pollen monitor OUYANG Yuhui;Zhang Deshan;ZHAO Liping;ZHANG Luo Journal of Capital Medical University    2012, 33 (6): 713-716.   DOI: 10.3969/j.issn.1006-7795.2012.06.001 Abstract （1451）      PDF（pc） （3129KB）（746）       Knowledge map    Save Objective To analyze the correlation between the counting results of Durham pollen sampler and automatic pollen monitor, discuss the advantages and defects of two instruments. Methods Durham sampler and KH3000-1 automatic pollen monitor were set up next to each other on the same altitude. Airborne pollen concentrations were measured at the same time. Results Durham sampler has the merit of low cost and can classify the granules that adhere to the sampling surface, but pollen counting using Durham sampler require much time and skilled labor; KH3000-1 automatic pollen monitor count pollen particle using laser light in real time for computer processing, without the counting using a microscope, but it is difficult to distinguish pollen species. Conclusion The onset and peak time of pollen concentration measured by two devices were consistent, the results of automatic pollen monitor affected by pollens particle size and meteorological factors. Related Articles | Metrics Cited: Baidu(1)