首都医科大学学报 ›› 2014, Vol. 35 ›› Issue (2): 214-218.doi: 10.3969/j.issn.1006-7795.2014.02.015

• 基础研究 • 上一篇    下一篇

α-突触核蛋白和硝基化α-突触核蛋白在不同年龄段食蟹猴结肠中的表达

杨斌1, 杨巍巍2, 李昕2, 刘承伟1, 于顺2   

  1. 1. 桂林医学院病理和病理生理学系, 广西桂林 541002;
    2. 首都医科大学宣武医院神经生物学研究室 北京市老年病医疗研究中心分子诊断实验室 教育部神经变性病重点实验室, 北京 100053
  • 收稿日期:2013-12-03 出版日期:2014-04-21 发布日期:2014-04-16
  • 基金资助:

    国家自然科学基金(81071014),国家重点基础发展计划(973)计划(2011CB504101),国家科技支撑计划课题(2012BAI10B03),北京市自然科学基金(7102076),首都卫生发展科研专项课题(首发2011-1001-01)。

Differential expressions of α-synuclein and nitrated α-synuclein in colonic in different ages of cynomolgus monkeys

Yang Bin1, Yang Weiwei2, Li Xin2, Liu Chengwei1, Yu Shun2   

  1. 1. Department of Pathology and Pathophysiology, Guilin Medical College, Guilin 541002, Guangxi Zhuang Autonomus Region, China;
    2. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Laboratory of Molecular Diagnosis, Beijing Geriatric Medical Research Center, Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China
  • Received:2013-12-03 Online:2014-04-21 Published:2014-04-16
  • Contact: 刘承伟,于顺 E-mail:chw-liu@163.com;yushun103@163.com
  • Supported by:

    This study was supported by the National Natural Science Foundation of China(81071014), National Basic Research and Development Program(973 Program) of China(2011CB504101), the National Science and Technology Support Program(2012BAI10B03), Natural Science Foundation of Beijing(7102076), the Capital Health Research and Development Project(Shoufa 2011-1001-01).

摘要:

目的 研究α-突触核蛋白(α-synuclein,α-syn)及硝基化α-突触核蛋白(nitrated α-synuclein,n-syn)在不同年龄段食蟹猴结肠中的表达。方法 免疫组织化学方法检测α-syn和n-syn在不同年龄段食蟹猴结肠中的表达。免疫荧光双标观察α-syn和n-syn的共定位。结果 α-Syn和n-syn在食蟹猴结肠中存在共定位。α-Syn和n-syn在不同年龄段食蟹猴结肠中均有表达,但α-syn和n-syn在老年食蟹猴表达量最高,在青年食蟹猴表达量最低。结论 α-Syn和n-syn在食蟹猴结肠中存在共定位,且在老年猴中表达最高。

关键词: α-突触核蛋白, 硝基化α-突触核蛋白, 食蟹猴

Abstract:

Objective To explore the expressions of α-synuclein(α-syn) and nitrated α-synuclein(n-syn) in colonic in different ages of cynomolgus monkeys. Methods Immunohis-tochemistry was applied to measure the expressions of α-syn and n-syn in colonic in different ages of cynomolgus monkeys. Double immunofluorescent labeling was used to examine the co-localization of α-syn and n-syn. Results α-Syn and n-syn were colocalized in colonic of cynomolgus monkeys. α-syn and n-syn were expressed in colonic in different ages of cynomolgus monkeys. However, α-syn and n-syn expressions were the highest in colonic of old monkeys and the lowest in colonic of young monkeys. Conclusion α-Syn and n-syn are colocolized in colonic of cynomolgus monkeys and are the highest in colonic of old cynomolgus monkeys.

Key words: α-synuclein(α-syn), nitrated α-synuclein(n-syn), cynomolgus monkeys

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