Differential changes of AMPA receptor GluR1 and GluR2 subunits synaptic targeting in the spinal cord dorsal horns in rats with incisional pain

doi:10.3969/j.issn.1006-7795.2013.05.001

Abstract

Abstract:

Objective To investigate the synaptic targeting changes of AMPA receptor GluR1 and GluR2 subunits in the spinal cord dorsal horns in rats with incisional pain.Methods Thirty-two adult male SD rats were randomly divided into four groups (n=8 in each): Group Ⅰ (control group), Group Ⅱ (3 h after incision), Group Ⅲ (1 d after incision), and Group Ⅳ (3 d after incision). The rats in Group Ⅱ-Ⅳ received right plantar incision and the cumulative pain scores (CPS) and paw withdrawal thresholds (PWT) were tested at 3 h, 1 d, and 3 d after incision, respectively. All rats were euthanized for harvesting L3-L6 spinal cord dorsal horns tissue ipsilateral to incision after pain behaviors were measured. Western blotting was used to test the expression of GluR1 and GluR2 in the crude synaptosomal membrane and total soluble fraction of spinal cord dorsal horns in each group (n=3). Western blotting was also used to test the Stargazin phosphorylation level (Ser240/241) in the spinal cord dorsal horns of each group (n=3). Results The CPSs peaked at 3 h after incision, and then was gradually decreased, but were a bit higher than those of in Group Ⅰ at 3d after incision. The PWTs in incisional groups were significantly lower than those of in Group Ⅰ. The expression of GluR1 in synaptosomal membrane was significantly increased in incisional groups as compared with Group Ⅰ and peaked in Group Ⅱ. In contrast, the expression of GluR1 in total solution of Group Ⅱ~Ⅳ has not significant changes compared to Group Ⅰ. The expression of GluR2 in synaptosomal membrane and total solution did not change among the groups. Compared with Group Ⅰ, Stargazin phosphorylation level of total solution in spinal cord dorsal horns in Group Ⅱ~Ⅳ has no significant differences. Conclusion Surgical injuries may induce the synaptic targeting of GluR1-containing subunit AMPA receptor into the synaptosomal membrane in the spinal cord dorsal horns, but not change the expression of GluR2-containing subunit AMPA receptor, which eventually increase GluR1/GluR2 ratio and Ca²⁺ permeable AMPA receptors in the synaptosomal membrane,and thus enhance the excitation of AMPA receptors in synaptosomal membrane which lead to the transduction of pain signals in spinal cord dorsal horns. Meanwhile, this experiment confirms, synaptic targeting of AMPA receptors assisted by Stargazin is not associated with the phosphorylation of Stargazin(Ser240/241).

Key words: α-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid receptor, synaptic trafficking, pain, surgery

CLC Number:

R614

SHI Rong, WANG Yun, GUO Ruijuan, WU Anshi, YUE Yun. Differential changes of AMPA receptor GluR1 and GluR2 subunits synaptic targeting in the spinal cord dorsal horns in rats with incisional pain[J]. Journal of Capital Medical University, 2013, 34(5): 639-645.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2013.05.001

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2013/V34/I5/639

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