Pathological analysis of liver in fatal influenza A (H1N1) virus infected cases

doi:10.3969/j.issn.1006-7795.2015.03.019

Abstract

Abstract:

Objective In this study, we observed the pathological characteristics of liver after influenza A (H1N1) virus infection, studied the expression of hemagglutinin (HA),nucleoprotein (NP),CD68 as well as the transforming growth factor β₁ (TGF-β₁) in the livers in the death cases, trying to discover the potential pathogenesis of the fatal H1N1. Methods Eight cases of fatal human influenza A (H1N1) infection, including 2 autopsy and 6 paramortem needle puncture biopsies were enrolled into the study. Histologic examination, special stain and immunohistochemical assays were carried out. Results In the hepatic cells band-like necrosis appeared. Micro-bubbled fatty change was observed in the hepatic cells of the pregnant women. Immunohistochemical study demonstrated the presence of nuclear protein(NP) and hemagglutinin (HA) virus antigens in macrophages. CD68 was positively expressed in hepatic kuffer' cells. TGF-β was positively expressed in macrophages and the expression rate was significantly higher than that of controls in zone of necrosis. Conclusion The study indicated that the pathological changes of influenza A (H1N1) virus infected patients were not limited to the respiratory tract, the liver was another target. Its mechanism might be related to the role of CD68 and TGF-β in the course of the disease.

Key words: influenza A (H1N1), liver, pathological characteristics, CD68, transforming growth factor β₁(TGF-β₁)

CLC Number:

Wang Jue, Dai Jie, Wang Daye, Lyu Fudong, Hu Zijing, Jin Ronghua, Qi Jing, Duan Xuejing. Pathological analysis of liver in fatal influenza A (H1N1) virus infected cases[J]. Journal of Capital Medical University, 2015, 36(3): 437-440.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.03.019

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I3/437

References

[1] Shieh W J, Blau D M, Denison A M, et al. 2009 Pandemic influenza a (H1N1):pathology and pathogenesis of 100 fatal cases in the united states[J]. Am J Pathology, 2010, 177(1):166-175.
[2] Shelke V N, Kolhapure R M, Kadam D,et al. Pathologic study of pandemic influenzaA (H1N1) 2009 cases from India[J]. Pathol Int, 2012, 62(1):36-42.
[3] Mauad T, Hajjar L A, Callegari G D, et al. Lung pathology in fatal novel human influenza A(H1N1) infection[J]. Am J Respir Crit Care Med, 2010, 181:72-79.
[4] 段雪晶,李勇,戴洁,等. 重症甲型H1N1流感八例呼吸系统病理学特征分析[J].中华病理学杂志,2011,40(12):825-829
[5] Harms P W, Schmidt L A, Smith L B, et al.Autopsy Findings in Eight Patients With Fatal H1N1 Influenza[J]. Am J Clin Pathol, 2010, 134: 27-35.
[6] Harms P W, Schmidt L A, Smith L B,et al.Autopsy Findings in eight patients with fatal H1N1 influenza[J]. Am J Clin Pathol, 2010, 134: 27-35.
[7] Calore E E, Uip D E, Perez N M. Pathology of the swine-origin influenza A (H1N1) flu[J]. Pathol Res Pract, 2011, 207(2):86-90.
[8] Arbit B, Gaultier C R, Schwarz E R. H1N1 virus infection associated with acute myocardial infarction in a young patient without coronary artery disease-first reported case[J]. Acta Cardiol, 2011, 66(6):807-810.
[9] Shieh W J, Blau D M, Denison A M, et al, 2009 Pandemic Influenza A (H1N1):Pathology and Pathogenesis of 100 Fatal Cases in the United States[J]. Am J Pathology, 2010, 177(1):166-175.
[10] Childs R A, Palma A S, Wharton S, et al. Receptor-binding specificity of pandem-ic influenza A (H1N1) 2009 virus determined by carbohydrate microarray[J]. Nature Biotechnol, 2009, 27:797-799.
[11] Amanjit Bal, Vikas Suri, Baijayantimala Mishra,et al. Pathology and virology findings in cases of fatal influenza A H1N1 virus infection in 2009-2010[J]. Histopathology, 2012, 60:326-335.
[12] 李杨,秦晓松. 甲型H1N1流感患者Th1/Th2细胞因子水平的观察[J]. 中国医科大学学报, 2012,41(8):753-755.

[1]	Zhang Ying, Liu Chaonan, Shi Honglin, Liu Fang, Chen Dexi, Shi Hongbo. Transcriptome analysis of lipid metabolization-related differential genes mediated by P53 apoptosis-stimulating protein 2 in alcoholic liver disease [J]. Journal of Capital Medical University, 2023, 44(1): 85-92.
[2]	Feng Ying, Shi Ke, Wang Xianbo. Establishment and validation of 2-year predictive scoring model for patients with liver cirrhosis complicated with superior mesenteric venous thrombosis [J]. Journal of Capital Medical University, 2023, 44(1): 107-114.
[3]	Lu Chang, Hu Jiangtao, Li Jinjiao, Jiang Haili. Clinical analysis of cesarean delivery with cesarean myomectomy in pregnant women with different types of uterine myomas [J]. Journal of Capital Medical University, 2022, 43(4): 653-657.
[4]	Liu Yao, Feng Ying, Ren Jie, Wang Xianbo. The influencing factors of abnormal liver function in patients with COVID-19 and its dynamic changes in different drug treatments [J]. Journal of Capital Medical University, 2022, 43(3): 433-439.
[5]	Chen Jianhong, Lin Xin, Feng Jinqiu, Ding Pengpeng, Wang Miaomiao, Lin Lin, Liu Hong, Wu Jing. Phenotypic analysis of hepatocyte-specific Eva1a/Tmem166 gene knockout mice [J]. Journal of Capital Medical University, 2021, 42(4): 568-574.
[6]	Kong Ming, Li Shuang, Geng Hua, Zhou Li, Xu Manman, Liu Jing, Chen Yu. Influencing factors of heparin anticoagulant effect in the treatment of double plasma molecular adsorption system [J]. Journal of Capital Medical University, 2021, 42(4): 647-652.
[7]	Sun Ximu, Wang Huaguang, Zhao Xin, Lyu Shaocheng, He Qiang, Liu Lihong. Risk factors of cytomegalovirus infection in patients undergoing liver transplantation [J]. Journal of Capital Medical University, 2021, 42(4): 664-671.
[8]	Jiang Haili, Zhou Jianxin, Xing Yu, Lu Chang. Effects of epidural analgesia on the outcomes of forceps delivery [J]. Journal of Capital Medical University, 2021, 42(2): 317-322.
[9]	Zhang Juxia, Wu Anshi. Analysis of the perioperative clinical efficacy of piggyback and classical non-bypass liver transplantation [J]. Journal of Capital Medical University, 2020, 41(6): 954-959.
[10]	Shan Shan, Cong Min. Devoted to the prevention and control of liver diseases——Professor Jia Jidong [J]. Journal of Capital Medical University, 2020, 41(5): 839-843.
[11]	Fang Da, Zhao Qian, Du Ning, Zhao Liying, Ma Liang, Cui Shichang. Analysis of radiation dose of radiology intervention for patients with liver cancer [J]. Journal of Capital Medical University, 2020, 41(3): 454-457.
[12]	Lu Jing, Li Qi, Zhu Xiaorong, Xie Rongrong, Yang Jinkui. Construction and preliminary application of conditional KCNH6-knockout mice [J]. Journal of Capital Medical University, 2020, 41(1): 8-13.
[13]	Yang Longyan, Wang Yan, Xu Yuechao, Cheng Yanan, Ma Yan, Zhao Dong. Effect of angiopoietin-like 4 in the diagnosis of nonalcoholic fatty liver [J]. Journal of Capital Medical University, 2020, 41(1): 31-34.
[14]	Li Muchan, Xu Chunjun, Wang Yutao, Wang Cong. Clinical study on improving the quality of life in patients with early and middle stages hepatocellular carcinoma [J]. Journal of Capital Medical University, 2019, 40(3): 352-357.
[15]	Zhao Mengpei, Li Aihong, Cui Ying, Che Niancong, Zhang Qiuyun, Du Yuqiong, Fu Xiuwen, Gao Lianyin. Effect of Qingdu Decoction on autophagy of intestinal epithelial cells in rats with severe liver injury [J]. Journal of Capital Medical University, 2019, 40(3): 429-433.