Abstract: Objective To evaluate the cervical pathological features of cytological negative(Pap-)/high-risk human papillomavirus (HR-HPV+) positive women.Methods This study recruited 1 128 women aged 20-79 years who had undergone cervical screening by liquid-based cytology and HR-HPV test (13 HR-HPV types) at Beijing Chaoyang Hospital during Jan 2014 to Dec 2015. Colposcopy and cervical biopsy were carried out in 506 women. Results The prevalence of HPV in Pap negative/HPV positive (Pap-/HPV+) women was different from that of all HPV positive population at the same time, and the proportion of HPV-16 was higher. Pathological features:506 patients received colposcopic biopsy, 247 cases (50.19%) were diagnosed as HSIL, while 7 patients were diagnosed as invasive cervical cancer. The incidence of HSIL was related to the age. The younger, the higher risk of HSIL. There was a significant negative correlation between the age and the rate (r=-0.254, P=0.000). HSIL incidence was associated with different types of HPV infection, with HPV-16 being the highest (64.7%). Compared with the control group, women infected by HPV-16, 18,31,33,52 and 58 in the Pap-/HPV+increased significantly the risk of HSIL lesions. Conclusion Overall, 50.19% of Pap-/HPV+women had significant cervical lesions. Pap-/HPV+women showed different epidemiological characteristics in HPV and were at high risk of HSIL lesions.

Key words: cervical pathology, human papillomavirus-genotyping, high grade squamous intraepithelial lesion