Function of ether-a-go-go related gene channel in pancreatic β-cells

doi:10.3969/j.issn.1006-7795.2019.01.008

Journal of Capital Medical University ›› 2019, Vol. 40 ›› Issue (1): 40-44.doi: 10.3969/j.issn.1006-7795.2019.01.008

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Function of ether-a-go-go related gene channel in pancreatic β-cells

Zhao Miaomiao^1,2,3, Yuan Shasha^2,3,4, Li Qi^1,2,3, Lu Jing^1,2,3, Huang Haixia^5,6, Yang Jinkui^1,2,3

1. Department of Endocrine and Metabolic Diseases, Beijing Tongren Hospital, Capital Medical University, Beijing 100730, China;
2. Beijing Key Laboratory of Diabetes Research and Care, Beijing 100730, China;
3. Beijing Diabetes Institute, Beijing 100730, China;
4. Department of Endocrine and Metabolic Diseases, Beijing Luhe Hospital, Capital Medical University, Beijing 101149, China;
5. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China;
6. Beijing Key Laboratory of Metabolic Disorders Related Cardiovascular Diseases, Capital Medical University, Beijing 100069, China

Received:2018-11-08 Online:2019-01-21 Published:2019-01-23
Supported by:
This study was supported by National Natural Science Foundation of China (81370946,81400824,81800688), Natural Science Foundation of Beijing (7131005) and Foundation of Beijing Tongren Hospital, Capital Medical University (TRYY-KYJJ-2016-013).

Abstract

Abstract: Objective To investigate the function of ether-a-go-go related gene (ERG) channel in pancreatic β-cells. Methods Whole-cell patch clamp technique was conducted to record the potassium currents and action potential in wild type (WT) and ERG knockout (KO) primary cultured pancreatic β-cells. Intra-peritoneal glucose tolerant test (IPGTT) was performed to measure the blood glucose levels in both WT and KO mice. Results KO pancreatic β-cells had decreased potassium currents and prolonged action potential duration 90% compared with WT ones. KO mice had better blood glucose levels during IPGTT. Conclusion ERG plays a role in the potassium currents and action potential in pancreatic β-cells, which may affect the blood glucose levels in vivo.

Key words: ether-a-go-go related gene, pancreatic β-cell, patch-clamp, blood glucose

CLC Number:

Zhao Miaomiao, Yuan Shasha, Li Qi, Lu Jing, Huang Haixia, Yang Jinkui. Function of ether-a-go-go related gene channel in pancreatic β-cells[J]. Journal of Capital Medical University, 2019, 40(1): 40-44.

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Function of ether-a-go-go related gene channel in pancreatic β-cells

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