Extraction process and determination of caffeine and catechins from tea

doi:10.3969/j.issn.1006-7795.2019.05.014

Abstract

Abstract: Objective Research on heating and ultrasonic method optimum extraction technology of caffeine (CAF),epigallocatechin gallate (EGCG), epicatechin gallate (ECG),epicatechin (EC) in tea. Methods The amounts of effective components from tea were determined by high performance liquid chromatography (HPLC) and the ethanol concentration, extraction time and solid-liquid ratio as the factors affecting were studied. Results Heating extraction process was ethanol concentration of 65%, solid-liquid ratio of 1:25 and extraction for 30 min at 85℃. Ultrasonic extraction process was ethanol concentration of 65%, solid-liquid ratio of 1:25 and ultrasonic for 60 min at room temperature. Conclusion The results showed that two kinds of extraction technology had the same extraction efficiency and four kinds of effective components in the extract content from the high to the end order were EC, EGCG, CAF and ECG. Catechin material in EC, EGCG and ECG in green tea and scented tea were significantly higher than that of black tea and puer tea.

Key words: tea, caffeine, catechins, heating extraction, ultrasonic extraction

CLC Number:

R284

Shao Jianqun, Li Qingyuan, Wang Yuji, Zhang Feng. Extraction process and determination of caffeine and catechins from tea[J]. Journal of Capital Medical University, 2019, 40(5): 738-743.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2019.05.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2019/V40/I5/738

References

[1] 王丽丽,陈键,宋振硕,等. 茶叶中茶多酚检测方法研究进展[J]. 茶叶科学技术,2013(4):6-12.
[2] 焦婵媛,景春晖,员小婷,等. 表儿茶素及其衍生物表儿茶素没食子酸酯通过MAPK-ERK44/42通路抑制前列腺间质细胞增殖[J].天津中医药,2017,34(3):200-203.
[3] 陈星卉.表儿茶素没食子酸酯抑菌活性的研究[J]. 黑龙江医药科学, 2017, 40(6):129-130,132.
[4] 蔡轶,魏沁,蔡跃鹏,等. 表儿茶素没食子酸酯对人鼻咽癌C666-1细胞凋亡的影响[J].华南师范大学学报:自然科学版, 2015,47(4):103-107.
[5] 雷梦侠,王丹丹,于飞,等. 表没食子儿茶素没食子酸酯相关肿瘤临床试验研究进展[J].中国肿瘤临床,2018,45(2):97-102.
[6] Makena P S, Chung K T. Effects of various plant polyphenols on bladder carcinogen benzidine-induced mutagenicity[J]. Food Chem Toxicol, 2007, 45(10):1899-1909.
[7] 黄鸿,夏晓艳,赵余庆. 茶叶中茶氨酸、咖啡因和茶碱检测方法的研究进展[J]. 沈阳药科大学学报,2018,35(8):696-706.
[8] 侯倩倩,曹雪姣,王嘉宝,等. 咖啡因预防阿尔兹海默病作用机制的研究[J]. 现代生物医学进展,2017,17(23):4452-4455.
[9] 刘洋,董文斌.咖啡因对早产儿支气管肺发育不良的预防作用[J].中国当代儿科杂志,2018,20(7):598-602.
[10] 刘寒旸,周艳,龚宇,等. 咖啡因在肥胖、糖尿病和肿瘤中的研究进展[J]. 医学综述,2016,22(5):928-932.
[11] 浦周芳,韩丽琴,曹宏梅.茶叶中茶多酚提取及含量测定的研究[J].食品研究与开发,2013,34(17):83-85.
[12] 闫洪军,郑择,李涵汶,等. 茶叶中咖啡因的提取及条件优化[J]. 安徽化工,2018,44(4):36-37.
[13] Sökmen M, Demir E, Alomar S Y. Optimization of sequential supercritical fluid extraction (SFE) of caffeine and catechins from green tea[J]. J Supercrit Fluids, 2018, 133, 171-176.
[14] 陈键,王丽丽,宋振硕.茶叶色素研究进展[J]. 茶叶科学技术,2013(4):13-16.
[15] 胡雅琼,罗理勇,曾亮.茶叶提取物对帕金森病的防治作用研究进展[J].中草药,2014,45(9):1342-1348.
[16] Mancini E, Beglinger C, Drewe J, et al. Green tea effects on cognition, mood and human brain function:a systematic review[J]. Phytomedicine, 2017, 34:26-37.
[17] 申雯,黄建安,李勤,等. 茶叶主要活性成分的保健功能与作用机制研究进展[J].茶叶通讯,2016.43(1):8-13.
[18] 高飞虎,李雪,张雪梅,等.茶叶的主要功效及在食品中的应用研究进展[J].南方农业,2018,12(28):38-40.