Enzyme Immunoassay of Thromboxane B2

Abstract

Abstract: An enzyme immunoassay for the measurement of TXB₂ Was developed. TXB₂ Was coupled with β-galactosidase by mixed anhydride reaction. The separation of immunocomplex from the free form of TXB₂ Was accompli-shed by the double antibody method. The precipitated enzyme activity was measured fluorometrically with 4-methyl-umbelliferyl-β-D-galactoside as substrate. The sensitivity of this method is <0.008pmol (<3.125pg) per tu-be. The inter-assay and intra-assay coefficients of variation (CV) were 5.9% and 5.4% respectively. TXB₂ during AA-induced aggregation of ra-bbit platelets Was directly measured, and regression analysis of the data comparing EIA (Y) and RIA (X) gave the equation Y=0.821X+1.397, cor-relation coefficient r=0.955.

Key words: enzyme immunoassay, thromboxane, platelet

Li Lin. Enzyme Immunoassay of Thromboxane B₂[J]. Journal of Capital Medical University, 1988, 9(1): 7-11.

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Enzyme Immunoassay of Thromboxane B₂

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