Abstract: In this paper the method of gas chromatographic(GC)analysis for eicosapentaenoic acid(EPA)and docosahexaenoic acid(DHA)in the anchovy oil was reported. The polyunsaturated fatty acids(PUFA)in the fish oil were saponified with 0.5 mol/L NaOH-CH₃OH, and esterified with BF₃-CH₃OH for GC analysis. The quantity was determined by internal standard method with TSA-M as a reference. In five determinations, the average rate of reclamation for EPA-M and DHA-M was 89.86%, 89.78% respectively. The variation coefficient for EPA-M was 0.80%, and for DHA-M was 0.64%. The proposed method is simple, rapid and reproducible.

Key words: gas chromatographic analysis, anchovy oil, EPA, DHA