Abstract:

The test used rats and polyacrylamie gel electrophoresis(PAGE)to analyze the LDH isoenzyme electrophoresis maps about the tissue specimen specificity and the changes in various age groups. The results showed that each tissue specimen had a characteristic LDH isoenzyme electrophoresis map. The characteristics reflected the difference of the regulation during and post transcription of A and B gene among different tissues. The results also showed that there were specified LDH isoenzyme electrophoresis maps in each development stage of each tissue. For example, in the tissue of skeletal muscle, the contents of five kinds of LDH isoenzyme were significantly different between the aged and infant rats. These changes of LDH isoenzyme may have a very important physiological significance during ontogenesis. It may cause changes in organ function.

Key words: LDH, isoenzyme, aged, electrophoresis