Abstract: In order to observe the effect of colony stimulating factor on proliferation of malignant cells of chronic myelogenous leukemia (CML) CD34⁺HLA DR⁺ cells which contain ph 1 chromosome and BCR/ABLrearrangement were separated from 8 CMLbone marrow sample by two step MoAb magnetical microbeads separating method,and the effect of colony stimulating factor(SCF IL-3 and Epo)on proliferation of this kind of cells were observed in vitro. The results indicated that elimination of malignant cells was delayed for 14 d in colony stimulating factor liquid culture as compared with liquid culture without colony stimulating factor(P<0.05).This observation indicates that colony stimulating factor may prolong the survival time of malignant cells of CMLin vitro.

Key words: chronic myelogenous leukemia, culture in vitro, colony stimulating factor