Abstract: To investigate the role of endogenetic NO in the pathogenesis of acute hemorrhagic and necrotic pancreatitis, AHNP was induced in rats (300～350g) by intraductal administration of 5% sodium taurocholate. The pathologic morphologic changes of pancreatic blood micro-vessel and acinus were observed under light microscope. The expression of eNOS in endothelium was examined through Immunohistochemistry and the Western blot analyses were performed on the expression of iNOS in tissue. The injuries were found in pancreatic micro-vessel 5 min after the inducement, accompanied by acinus coagulation necrosis. These changes were aggravated with the development of the disease. The expression of eNOS in endothelium of pancreatic micro-vessel began to increase significantly 10 min after the administration (P<0.05) and continue to increase with the development of the disease. The significant increase of the expression of iNOS in pancreatic tissue began at 5 min after the administration (P<0.05) and continued to 6 h after the inducement.

Key words: acute hemorrhagic and necrotic pancreatitis, NO, NOS