Abstract: To explore tubulin-β expression of normal and tumor cells in vitro, 3T3, BGC-823, SH-SY5Y, C₆, BT325 were cultured in vitro and immunofluorescent staining was used to investigate tubulin-β immunoreactivity. Results showed that the po sitive cells were intense red in body and processes which presented in all of cells. The findings prove that tubulin-β expression may occur in any multipl ication cell including normal cells and tumor cells.

Key words: tubulin-β, immunofluorescence, multiplication