Abstract: The propofol in human blood was measured by HPLC with fluorimeter. Thymol was used as an internal standard. Following the extraction with hexane, propofol was extracted from the hexane into the NaOH methanol solution(0.4 mol/L). Chromatographic condition: a C18 ODS column(250 mm×4.6 mm, 5 μm) was used; the mobile phase was a mixture solution of acetonitrile and water (60%,40%, added 1 mL of trifluoroacetic acid per 1 000 mL); the excitation and emission wave lengths were 276 and 310 nm respectively. The limit of quantification is 0.5 μg/L, average recovery is between 93% and 104%, RSD <9.2%.

Key words: propofol, concentration in blood, HPLC