Journal of Capital Medical University ›› 2004, Vol. 25 ›› Issue (4): 472-474.

• 论著·临床研究 • Previous Articles     Next Articles

Lishi No.5 Formula Capsule on Neurotoxicity Induced by Aβ

Zhang Jingyan1, Wang Rong1, Li Zizhong2, Ji Zhijuan1, Cheng Haoran2, Li Shenghai2, Sheng Shuli1   

  1. 1. Beijing Research Laboratory for Brain Aging, Xuanwu Hospital, Capital University of Medical Sciences;2. Guangdong Gao Ming Encephalopathy Medical and Pharmacological Academy
  • Received:2003-10-24 Revised:1900-01-01 Online:2004-10-15 Published:2004-10-15

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Abstract: The aim was to observe the the effect of Lishi No.5 Formula capsule on the growth of human neuroblastoma cell line SY5Y and on the neurotoxicity induced by Aβ. Aβ25-35 was synthesized by solid phase method and purified by HPLC. Lishi No.5 Formula water extract was 0.5 g/mL. Human neuroblastoma cells SY5Y were segregated into 3 groups: normal control group; Aβ25-35(25 μmol/L) damaged group; and Aβ25-35(25 μmol/L) + Lishi No.5 Formula water extract (corresponding to Lishi No.5 Formula 0.5 g/L culture medium) for neuroprotection group. MTT metabolic rate, LDH leakage rate, axonal length, area of cell body were used as indicators. Compare with normal control group, Aβ25-35 damaged group showed reduced MTT metabolic rate, increased LDH leakage rate, and diminished axonal length and area of cell body, but the addition of Lishi No.5 Formula normalized the foregoing changes. Lishi No.5 Formula has neuroprotective effect. It can diminish neurotoxicity of Aβ.

Key words: Lishi No.5 Formula, Aβ25-35, neurotrophic effect, human neuroblastoma cell line SY5Y

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