Abstract:

Objective To investigate whether or not survival was decreased after incubation with high glucose concentrations in culture media, and to examine the effects of glucose on the expression of intercellular adhesion molecule-1(ICAM-1)、vascular cell adhesion molecule(VCAM-1) in cultured human umbilical vein endothelial cells. Methods Human umbilical vein endothelial cells (ECV304) were incubated in a culture medium with 11.2 mmol/L, 16.8 mmol/L and 33.6 mmol/L glucoseconcentrations for 24 h, 48 h, 72 h respectively. The trypan-blue exclusion test was used to study the influence of D-glucose on cell survial.Theexpression of intercellular adhesion molecule-1(ICAM-1), vascular cell adhesion molecule-1(VCAM-1) were measured by flowcytometry. Results When D-glucose concentrations rose trypan-blue excluded cells were decreased. HUVEC exposed to a high glucose concentration (33.6 mmol/L) showed a 1.22-fold increase and a 1.27-fold increase in cell surface expression of ICAM-1 after 48 h and 72 h exposure compared with those cultured in medium with a low glucose concentration( 5.6 mmol/L). Conclusion High concentration of glucose can arrest the proliferative response and show that even a short-term exposure of endothelial cells (ECs) to high glucose concentration leads to their activation associated with increased expression of adhesion molecules such as ICAM-1 and that this effect may appears more significantly along with time of exposuring high concentration of glucose.

Key words: endothelial cell, glucose concentrations, adhesion molecules