Abstract:

Objective The hypothesis was under investigation that verapamil as a Calcium-channel antagonist, is introduced to prevent excretion of anticancer drugs from cells excerting reversing effects on drug resistance in bladder cancer. Methods MTT and HPLC method are applied to detect the alteration on the sensibility of cancer cells to anticancer drugs before and after introducing verapamil. Results Verapamil has no effect on the growth of bladder cancer-sensible cells(EJ). For the drug-resisted bladder cancer cells (EJ-R), effects of anti-cancer drugs are enhanced with the increasing concentration of verapamil. Conclusion Glucoprotein P-GP_ 170 on the membranes of bladder cancer cells can excrete anti-cancer drugs from the cells against concentration gradient. Verapamil blocks Calcium channels thereby reduce excretion of anti-cancer drugs by P-GP₁₇₀. By guaranteening the concentration of anti-cancer drugs in the cancer cells, drug resistance of cancer cells is reversed.

Key words: bladder cancer, drug resistance, muti-drug resistance gene