Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (4): 428-431.

Previous Articles Next Articles

Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

Jia Hongxiao^1,3, Ma Xin¹, Jiang Yong², Ruan Yan³, Zhang Jizhi¹, Tu Pengfei², Zhang Dai³

1. Beijing Anding Hospital, Capital University of Medical Sciences;2. School of Pharmaceutical SciencesPeking University;3. Mental Health Institute, Peking University

Received:2005-06-30 Revised:1900-01-01 Online:2005-08-24 Published:2005-08-24

Abstract

Abstract:

Objective To investigate the inhibitory effect of tenuigenin on beta amyloid protein(Aβ) which caused Alzheimer's Disease(AD).Methods Western blotting was used to test the Aβ and C99 levels of cells; MTT and LDH was used to test the neurotoxicity of tenuigenin to the cells.Results Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, didn't have neurotoxicity to cells; it can decrease Aβ and C99 levels of SH-SY5Y APP695 cells, but it didn't decrease Aβ and C99 levels of SH-SY5Y SPA4CT cells.Conclusion Tenuigenin can inhibit the production of beta amyloid protein of SH-SY5Y APP695 cells, it's mechanism may come through affecting the β-secretase proteolytic activities.

Key words: Alzheimer disease(AD), amyloid &beta, -peptides(A&beta, ), C99, Western blotting

CLC Number:

R749.0+6

Jia Hongxiao;Ma Xin;Jiang Yong;Ruan Yan;Zhang Jizhi;Tu Pengfei;Zhang Dai. Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA[J]. Journal of Capital Medical University, 2005, 26(4): 428-431.

[1]	Jiang Lingling, Ruan Xiangyan, Li Yanqiu, Zhang Mingzhen, Wang Zecheng, Jin Jing, Tian Xuanxuan, Liu Anming, Alfred O. Mueck. Comparison of ethinyl-estradiol/drospirenone tablets alone or in combination with orlistat on ovulation recovery rate and metabolic indices in overweight/obese patients with polycystic ovary syndrome [J]. Journal of Capital Medical University, 2025, 46(4): 607-613.
[2]	Ma Jun, Ruan Xiangyan, Xu Zhongting , Zhao Yue , Wang Yuejiao, Alfred O.Mueck, . Clinical efficacy of letrozole combined with Zi Gui Nv Zhen Capsules in patients with PCOS complicated by thin endometrium-induced infertility [J]. Journal of Capital Medical University, 2025, 46(4): 632-636.
[3]	Guo Yibing, Meng Ran. Research advances on lipoprotein(a) in cerebrovascular diseases [J]. Journal of Capital Medical University, 2025, 46(1): 71-75.
[4]	Zhang Xiao, Zhang Wei, Wang Zunli, Wen Nuan, Liu Conghui, Li Zhongxin. Analysis of influencing factors of time prolongation of time up and go test in maintenance hemodialysis patients [J]. Journal of Capital Medical University, 2025, 46(1): 130-135.
[5]	Liu Yang, Gao Chao, Wang Xiaojie, Zhang Liang, Wu Anshi. Effect of remimazolam and propofol on postoperative delirium in elderly patients undergoing cerebral endovascular surgery [J]. Journal of Capital Medical University, 2024, 45(6): 1023-1028.
[6]	Wu Anqi, Luo Zeni, Jiang Yao, Wang Zhikai, Zhu Xinping, Sun Ximeng. Exploratory study on the ameliorative effects of Trichinella spiralis paramyosin on chronic inflammatory bowel disease [J]. Journal of Capital Medical University, 2024, 45(5): 891-899.
[7]	Wu Zhaozhen, Zheng Hua, Sheng Shuyan, Zhang Quan, Zhang Hui, Li Jie, Lyu Jialin, Qian Zhe, Wang Shouzheng, Li Xi , Hu Ying. Observation on the preliminary efficacy of neoadjuvant immunotherapy combined with chemotherapy in non-small cell lung cancer [J]. Journal of Capital Medical University, 2024, 45(4): 642-648.
[8]	Li Yue, Li Man, Zuo Long, Yang Lei, Qin Wei, Hu Wenli. Relationship between orthostatic hypotension and summary cerebral small vessel disease score [J]. Journal of Capital Medical University, 2024, 45(3): 508-514.
[9]	Jin Ruina, Bian Haibo, Zhang Xiaomin, Yang Fan, Wang Wanping. Construction and activity identification of anti-OX40/anti-EGFR bispecific antibody [J]. Journal of Capital Medical University, 2024, 45(2): 296-301.
[10]	Li Xiaotong, Zhao Xiaobin, Lyu Ruijuan, Yuan Leilei, Chen Qian, Wang Qun, Ai Lin. 18F-FDG PET/CT imaging features of autoimmune encephalitis with anti-LGI1 antibody [J]. Journal of Capital Medical University, 2024, 45(1): 8-12.
[11]	Guo Yuehong, Huang Jingwei, Zhang Teng, Yang Minfu. Factors associated with preoperative ^99mTc-MIBI SPECT/CT in patients with suspected primary hyperparathyroidism [J]. Journal of Capital Medical University, 2024, 45(1): 31-35.
[12]	Yang Yu, Qiao Hongwen, Shuai Dongmei, Cui Chunlei, Ma Suwen, Zhang Chun, Lu Jie. Application value of standardized nursing in multi-tracers imaging of nuclear medicine department [J]. Journal of Capital Medical University, 2024, 45(1): 42-45.
[13]	Jiang Yumei, Zhang Ziwei, Wang Jitian, Yang Yu. Effect of nitric oxide donor drugs on the antibacterial activity of Porphyromonas gingivalis in vitro [J]. Journal of Capital Medical University, 2024, 45(1): 104-110.
[14]	Tian Xiaoru, Qian Kun, Zhang Peilong, Zhang Yi. Analysis of risk factors for postoperative complications after uniportal-VATS lobectomy or segmentectomy in elderly patients with lung cancer [J]. Journal of Capital Medical University, 2024, 45(1): 118-126.
[15]	Zhuang Jiamian, Zeng Yuran, Chen Shaoqi. Evaluation of the mechanical properties of supraspinatus tendons in hemodialysis patients with high frame rate sound touch elastography [J]. Journal of Capital Medical University, 2023, 44(6): 936-940.

Inhibitory Effect of Tenuigenin on Beta Amyloid Protein Production of SH-SY5Y Cells Transfected with APP695 cDNA

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments