The Effects of LPS on Expression of mCD14 in THP-1 Cell Line

Journal of Capital Medical University ›› 2005, Vol. 26 ›› Issue (6): 711-713.

• 基础研究 • Previous Articles Next Articles

The Effects of LPS on Expression of mCD14 in THP-1 Cell Line

Hou Benxiang¹, Zhang Chen¹, Yin Jun²

1. Faculty of Stomatollogy, Capital University of Medical Sciences;2. Institute of Microbiology andEpidemiology, Academy of Military Science

Received:2005-01-15 Revised:1900-01-01 Online:2005-12-24 Published:2005-12-24

Abstract

Abstract: Objective To investigate the effects of LPSon expression of mCD14 on the THP-1 cell line.Methods Under vitro cultured conditions human THP-1 cell line was stimulated respectively by 0(as control), 0.1～50 mg/L LPSfor 24 h, the cell apoptosis rate was determined with flow cytometry.The contents of TNFa and IL-6 in the supernatant were measured by ELISA.The expression levels of mCD14 on the cell membrane were measured in the levels of relative density of PCRproducts.Results After activation of LPS, the cell apoptosis rate was promoted and levels of TNF-α and IL-6 in culture supernatant were remarkablely increased.The mCD14 expression on the cell membrane was significantly up-regulated.Conclusion LPScan lead to activition of THP-1 and significantly up-regulate the expression of CD14mRNA.It reveals that LPSmay be an important inflammatory factor on THP-1 cell reactions.

Key words: LPS, CD14, TNFα, IL-6, THP-wash basin

CLC Number:

R392

Hou Benxiang;Zhang Chen;Yin Jun. The Effects of LPS on Expression of mCD14 in THP-1 Cell Line[J]. Journal of Capital Medical University, 2005, 26(6): 711-713.

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The Effects of LPS on Expression of mCD14 in THP-1 Cell Line

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