Abstract: Objective To determine the prevalence of prothrombin G20210 gene polymophism in Chinese Han population and investigate whether it is associated with the risk of pulmonary thromboembolism(PTE).Methods One hundred one PTE patients and 101 age and sex-matched healthy controls from the same geographic area were enrolled in the study.All patients were diagnosed on the basis of lung ventilation/perfusion scan and/or multi-slice CT pulmonary angiography(CTPA) as well as medical history and clinical manifestations.Genome DNA was extracted from whole blood using KI-phenol-chloroform.Polymerase chain reaction(PCR),restriction enzyme digestion and polyacrylamide Gel Electrophoresis(PAGE) were used to identify the G20210A mutation.Results Prothrombin gene G20210A mutation was not found in both PTE patients and controls.Conclusion Prothrombin gene G20210A mutation is not associated with PTE risk in Chinese Han populations.

Key words: pulmonary thromboembolism, gene polymorphism, single nucleotide polymorphism