Abstract: Objective To investigate the interaction between the G894T polymorphism of endothelial nitric oxide synthase(eNOS) gene and the C825T polymorphism of GNB3 in the way of sunergistic effects for essential hypertension(EH).Methods Polymerase chain reaction combined with restriction enzyme digestion was used to detect the G894T polymorphism of eNOS gene and C825T polymorphism of GNB3 gene in 151 normotensive controls and 310 hypertensive patients.Results The frequencies of T allele and GT+TT genotype of eNOS gene G894T polymorphism in EH were significantly higher than in control group;There were no significant difference of the GNB3 gene C825T polymorphism CC,CT,TT genotypes and C,Talleles between hypertensive patients and normotensive controls;The odds ratio(OR) estimated by combined analysis with eNOS 894T and GNB3 825T(1.77) was markedly increased compared with that estimated alone from either eNOS 894T allele(0.53) or GNB3 825T allele(1.05).Conclusion eNOS 894T allele should be a risk factor for EH in chinese Han nationality,there was a significant synergistic effects of eNOS 894T allele and GNB3 825T allele in EH.

Key words: gene polymorphism, essential hypertension, synergistic effects, G protein, endothelial nitric oxide synthese(eNOS)