Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

Journal of Capital Medical University ›› 2007, Vol. 28 ›› Issue (1): 68-70.

• 基础研究 • Previous Articles Next Articles

Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

Zhang Jun¹, Zhang Zhongtao¹, Wang Yu¹, Wang Ping², Li Jianshe¹, Zhou Yanzhong¹

1. Department of General Surgery, Beijing Friendship Hospital, Capital Medical University;2. Department of Pathophysiology, Beijing Friendship Hospital, Capital Medical University

Received:2006-05-22 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24

Abstract

Abstract: Objective To study the effects of endothelin-1 and its receptor antagonists on the expression of collagen-1 mRNA in HSC-T6 cells. Methods RT-PCR analysis was used in the research. Results The expression of collagen-1 mRNA in ET-1 and ET-1+BQ-123 group was significantly higher than that of control group (0.706±0.027 and 0.724±0.059 vs 0.400±0.047, P<0.05). The expression in ET-1+BQ-788 group and ET-1+BQ-123+BQ-788 group was significantly lower than ET-1 group (0.427±0.131 and 0.382±0.022 vs 0.706±0.027, P<0.05). Conclusion ET-1 can induce high expression of collagen-1 mRNA in HSC. BQ-788, and the selective ETB receptor antagonist, can inhibit high expression of collagen-1 mRNA in HSC-T6 induced by ET-1.

Key words: endothelin-1, hepatic stellate cell, collagen type 1, RT-PCR

CLC Number:

R575.2

Zhang Jun;Zhang Zhongtao;Wang Yu;Wang Ping;Li Jianshe;Zhou Yanzhong. Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells[J]. Journal of Capital Medical University, 2007, 28(1): 68-70.

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Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

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