Abstract: Objective To clone human single chain antibodies(scFv) against keratin from a large phage antibody library and analyze the specificity of antigen binding activity and the homology of gene sequences. Methods A human large phage antibody library was panned for four times. The specificity of the antigen binding activity of the selected clones was identified by ELISA. Positive clones were analyzed by DNA fingerprint and sequencing. Results After 4 rounds of "adhesion-elution-amplification" panning, 9 clones expressing anti-keratin scFv were obtained. DNA fingerprint and sequence analysis showed that they had different antibody V region encoding genes. Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research.

Key words: phage antibody libraries, keratin, scFv