Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

Journal of Capital Medical University ›› 2007, Vol. 28 ›› Issue (1): 68-70.

• 基础研究 • Previous Articles Next Articles

Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

Zhang Jun¹, Zhang Zhongtao¹, Wang Yu¹, Wang Ping², Li Jianshe¹, Zhou Yanzhong¹

1. Department of General Surgery, Beijing Friendship Hospital, Capital Medical University;2. Department of Pathophysiology, Beijing Friendship Hospital, Capital Medical University

Received:2006-05-22 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24

Abstract

Abstract: Objective To study the effects of endothelin-1 and its receptor antagonists on the expression of collagen-1 mRNA in HSC-T6 cells. Methods RT-PCR analysis was used in the research. Results The expression of collagen-1 mRNA in ET-1 and ET-1+BQ-123 group was significantly higher than that of control group (0.706±0.027 and 0.724±0.059 vs 0.400±0.047, P<0.05). The expression in ET-1+BQ-788 group and ET-1+BQ-123+BQ-788 group was significantly lower than ET-1 group (0.427±0.131 and 0.382±0.022 vs 0.706±0.027, P<0.05). Conclusion ET-1 can induce high expression of collagen-1 mRNA in HSC. BQ-788, and the selective ETB receptor antagonist, can inhibit high expression of collagen-1 mRNA in HSC-T6 induced by ET-1.

Key words: endothelin-1, hepatic stellate cell, collagen type 1, RT-PCR

CLC Number:

R575.2

Zhang Jun;Zhang Zhongtao;Wang Yu;Wang Ping;Li Jianshe;Zhou Yanzhong. Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells[J]. Journal of Capital Medical University, 2007, 28(1): 68-70.

[1]	YUAN Yan-peng;GUAN Yun-qian;LIN Qing-ling;XUE Jin-hua;CAI Yan-ning. Analysis of key clock genes expression in individual cells with multiplex nested reverse transcriptase-PCR [J]. Journal of Capital Medical University, 2011, 32(3): 365-370.
[2]	CHEN Hui;MA Chao;ZHANG Yu-xian;MA Hong. Adiponectin Modulates MMP-13 and TIMP-1 Expression via p38MAPK Pathway in Rat Hepatic Stellate Cell Line [J]. Journal of Capital Medical University, 2010, 31(3): 304-309.
[3]	XU Hong-tao;GAO Lian-ru;ZHANG Ning-kun;ZHU Zhi-ming;FEI Yu-xing;YANG Ye;WANG Zhao-jun;LIU Ying-ming;CAO Yi;CHEN Yu;LI Xian-feng. Effect of Autologous Mononuclear Bone Marrow Cell Implantation on the Plasm Level of Urotensin-Ⅱ and Endothelin-1 in Patients with Acute Myocardial Infarction [J]. Journal of Capital Medical University, 2010, 31(1): 99-102.
[4]	BAI Yan-feng;CONG Min;WANG Ping;LIU Tian-hui;WU Xiao-ning;YANG Ai-ting;TANG Shu-zhen;MA Hong;JIA Ji-dong;YOU Hong. Suppression of Tissue Inhibitor of Metalloproteinase-1 Gene Expression by Recombinant Lentivirus Carrying Small Interfering RNA of TIMP-1 in Rat Hepatic Stellate Cell [J]. Journal of Capital Medical University, 2009, 30(3): 309-312.
[5]	LIU Tian-hui;CONG Min;WANG Ping;YOU Hong. Overexpression of MMP-13 Inhibited Collagen I Expression in Rat Hepatic Stellate Cells [J]. Journal of Capital Medical University, 2009, 30(3): 313-316.
[6]	WANG Yu;LIU Tian-hui;JIA Ji-dong;HU Zhong-jie. In vitro Study on the Infectivity of Hepatitis B Virus on Human Hepatic Stellate Cells [J]. Journal of Capital Medical University, 2009, 30(3): 321-325.
[7]	Ji Xiujuan;Xu Jian;Zhang Xuelian;Guo Limin;Yang Jinkui. Expression of ACE-related Carboxypeptidase(ACE2) in Human and Rodent Pancreata [J]. Journal of Capital Medical University, 2007, 28(3): 278-282.
[8]	Gong Hao;Wang Yu;Zhang Zhongtao;Li Jianshe;Ma Xuemei;Zhou Yanzhong. Effects of AngiotensinⅡ and Its Receptor Antagonist on CollagenⅠSynthesis of Hepatic Stellate Cells [J]. Journal of Capital Medical University, 2007, 28(1): 7-9.
[9]	Cong Min;Wang Ping;Liu Tianhui;Xu Yong;Lu Yan;Tang Shuzhen;Liu Xiaoming;Wang Baoen;Jia Jidong;You Hong . Suppression of Tissue Inhibitor of Metalloproteinase-1 on Gene Expression by Small Interfering RNA in Rat Hepatic Stellate Cell [J]. Journal of Capital Medical University, 2006, 27(6): 767-770.
[10]	Li Li;Gao Xiulai;Song Yizhi;Lu Tao;Jing Peng . Reconstruction and Expression of Regucalcin Fusion Protein in E.coli [J]. Journal of Capital Medical University, 2006, 27(6): 788-791.
[11]	Zhang Peng;Ma Hong;Wang Liting;Jia Jidong. Dynamic Expression of OB-Rb in CCl₄ Induced Hepatic Fibrosis in Rats [J]. Journal of Capital Medical University, 2006, 27(4): 464-468.
[12]	Zhang Juan;Ding Huiguo;You Hong;Tang Shuzhen;Shang Hongwei;Wang Xingcui;Jia Jidong. Expressions of Subtypes of Somatostatin Receptors in Activated Human Hepatic Stellate Cells and Its Clinical Significance [J]. Journal of Capital Medical University, 2006, 27(4): 441-444.
[13]	You Hongjie;Wu Xiaotao;Pan Ying;Wang Zesheng. Recombination and Expression of Chromogranin B Fusion Protein in E. Coli [J]. Journal of Capital Medical University, 2006, 27(2): 206-209.
[14]	Cai Yanning;Zuo Xiaohong;Liu Shu;Xie Shu;Wen Mei;Chen Biao. Expression Profiling of Per1 Gene in Mouse Striatum Detected Using Real Time RT-PCR [J]. Journal of Capital Medical University, 2006, 27(1): 63-65.
[15]	Zhang Liqun;Chen Hangwei;Wang Sihai;Xin Qinghong;Du Yuguo;You Lanhua. Construction of an Eukaryotic Cell Expressing Vector of hRSV M2-1 Gene and its Transfection into Lung Cancer Cell PAa and A549 [J]. Journal of Capital Medical University, 2005, 26(6): 733-736.

Effect of Endothelin-1 and Its Receptor Antagonists on the Expression of Collagen-1 mRNA in HSC-T6 Cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments