Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance

doi:10.3969/j.issn.1006-7795.2009.06.012

Journal of Capital Medical University ›› 2009, Vol. 30 ›› Issue (6): 780-783.doi: 10.3969/j.issn.1006-7795.2009.06.012

• 慢性丙型肝炎临床研究 • Previous Articles Next Articles

Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance

LIU Ya-li¹, SHI Ying², MA Li-na¹, JI Yun-xia², WEI Fei-li², WU Hao², CHEN De-xi², CHEN Xin-yue¹

1. International Medicine Ward, Beijing YouAn Hospital, Capital Medical University;2. STD/AIDS Research Laboratory, Beijing YouAn Hospital, Capital Medical University

Received:2009-09-22 Revised:1900-01-01 Online:2009-12-21 Published:2009-12-21

Abstract

Abstract: Objective To study the phosphorylation of K18 in apoptosis of HepG2 cells and its significance. Methods Apoptosis was induced in HepG2 cells by cisplatin(CDDP). Cells were stained by Annexin V and PI, then analyzed by flow cytometry to evaluate apoptosis. Protein levels of K18 and Ser33, Ser52 phosphorylation of keratin 18 in apoptosis cells were analyzed by immunofluorescence and Western blotting. Results The apoptosis rate of HepG2 cells increased along with the increase of CDDP concentration. The protein level of Ser52 phosphorylated keratin 18 increased along with the concentration of CDDP. The protein level of Ser33 phosphorylated keratin 18 increased with low concentration of CDDP, but decreased evidently with high concentration of CDDP. Conclusion Ser33, Ser52 phosphorylation of keratin 18 has close relationship with apoptosis of HepG2 cells.

Key words: keratin 18, phosphorylation, apoptosis

CLC Number:

R 735.7

LIU Ya-li;SHI Ying;MA Li-na;JI Yun-xia;WEI Fei-li;WU Hao;CHEN De-xi;CHEN Xin-yue. Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance[J]. Journal of Capital Medical University, 2009, 30(6): 780-783.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2009.06.012

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2009/V30/I6/780

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Phosphorylation of Keratin 18(Ser33, Ser52) in Apoptosis of HepG2 Cells and Its Significance

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