Abstract:

Objective To study the clinical application of DNA chip for common Gram-positive bacteria identification and drug resistance detection. Methods 445 Gram-positive strains were identified with physiological and biochemical methods, and were detected of drug resistance with diffusion disk method for susceptibility test. Then the strains were blindly detected with using DNA chip for identification and drug resistance. The data were analyzed using SPSS 10.0 for Windows software. Results The sensitivity of all identification indexes of DNA chip were higher than 96%, and all of their specificity were higher than 98%. The sensitivity of all drug resistance indexes of DNA chip were higher than 94%, and all of their specificity were higher than 90%. The chi square test values of all indexes were higher than 0.05, showing that there was no significant difference between DNA chip and the clinical routine methods. The Kappa values of all indexes were higher than 0.75, indicating that the coincidence between the two kinds of methods was good. Conclusion There is clinical potential of gene chip technology for bacteria identification and drug resistance detection.

Key words: bacteria identification, drug resistance, gene chip